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Xylooligosaccharides production from a sugarcane biomass mixture: Effects of commercial enzyme combinations on bagasse/straw hydrolysis pretreated using different strategies.
Ávila, Patrícia F; Franco Cairo, João Paulo L; Damasio, André; Forte, Marcus B S; Goldbeck, Rosana.
Afiliação
  • Ávila PF; Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Egineering, University of Campinas - UNICAMP, Campinas, SP, Brazil.
  • Franco Cairo JPL; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
  • Damasio A; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
  • Forte MBS; Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Egineering, University of Campinas - UNICAMP, Campinas, SP, Brazil.
  • Goldbeck R; Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Egineering, University of Campinas - UNICAMP, Campinas, SP, Brazil. Electronic address: goldbeck@unicamp.br.
Food Res Int ; 128: 108702, 2020 02.
Article em En | MEDLINE | ID: mdl-31955780
Xylooligosaccharides (XOS) are non-digestible food ingredients with prebiotic properties for selectively promoting the growth of probiotics, which provide many health benefits and several applications in the food and pharmaceutical industry. The objective of this study was to optimize the concentration of commercial hemicellulases for the production of XOS, with a 2-6 polymerization degree, using a mixture of sugarcane bagasse and straw pretreated with ionic liquid or diluted sulfuric acid. The concentrations of enzymes endo-1,4-xylanase (NS50030, Novozyme®) and α-L-arabinofuranosidase (GH51) (Megazyme®) were optimized using a central composite rotatable design (CCRD). The xylooligosaccharides (XOS) released by hydrolysis were analyzed via capillary electrophoresis and quantified with HPAEC-PAD. The XOS profile obtained from the hydrolisis of the pretreated sugarcane biomass mixture (MPSA) was similar to that obtained with the hydrolisis of MBX, which provided higher xylobiose (X2) concentration. Our results also demonstrated that pretreatment with an ionic liquid favored the requirement of lower enzyme concentration in enzymatic hydrolysis for having provided a biomass with lower lignin content than the pretreatment with dilute sulfuric acid. It required up to 20% less of the optimum concentration of the endo-1,4-xylanase mixture to achieve similar values to those obtained with the biomass pretreated with dilute sulfuric acid, representing a possible alternative to reduce enzymatic cost.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Celulose / Caules de Planta / Saccharum / Endo-1,4-beta-Xilanases / Glucuronatos / Glicosídeo Hidrolases Idioma: En Revista: Food Res Int Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Canadá
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Celulose / Caules de Planta / Saccharum / Endo-1,4-beta-Xilanases / Glucuronatos / Glicosídeo Hidrolases Idioma: En Revista: Food Res Int Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Canadá