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Quercetin repressed the stress response factor (sigB) and virulence genes (prfA, actA, inlA, and inlC), lower the adhesion, and biofilm development of L. monocytogenes.
Vazquez-Armenta, F J; Hernandez-Oñate, M A; Martinez-Tellez, M A; Lopez-Zavala, A A; Gonzalez-Aguilar, G A; Gutierrez-Pacheco, M M; Ayala-Zavala, J F.
Afiliação
  • Vazquez-Armenta FJ; Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico.
  • Hernandez-Oñate MA; CONACYT - Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico.
  • Martinez-Tellez MA; Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico.
  • Lopez-Zavala AA; Departamento de Ciencias Quimico Biologicas, Universidad de Sonora, Blvd. Rosales y Luis Encinas, Hermosillo, Sonora, 83000, Mexico.
  • Gonzalez-Aguilar GA; Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico.
  • Gutierrez-Pacheco MM; Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico.
  • Ayala-Zavala JF; Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Col. La Victoria, Hermosillo, Sonora, 83304, Mexico. Electronic address: jayala@ciad.mx.
Food Microbiol ; 87: 103377, 2020 May.
Article em En | MEDLINE | ID: mdl-31948618
The present study explored the effect of quercetin on the expression of virulence genes actA, inlA, inlC, and their regulatory components, sigB and prfA, in L. monocytogenes. Furthermore, the physicochemical changes on the surface, membrane permeability, and biofilm formation of quercetin-treated bacteria were evaluated. An inhibitory dose-dependent effect of quercetin (0.1-0.8 mM) was observed on the cell attachment on stainless steel at 2 and 6 h at 37 °C. Quercetin at 0.8 mM prevented the biofilm formation on stainless steel surfaces after 6 h of incubation at 37 °C, while the untreated bacteria formed biofilms with a cell density of 5.1 Log CFU/cm2. The microscopic analysis evidenced that quercetin at 0.2 mM decreased the biovolume and covered area of the attached micro-colonies. Also, sigB, prfA, inlA, inlC, and actA genes were downregulated by 7-29 times lower compared to untreated bacteria. In addition, quercetin decreased the superficial cell charge, increased the membrane permeability, and its surface hydrophobicity. These results demonstrated that quercetin prevented biofilm formation, repressed the genes of stress and virulence of L. monocytogenes and also altered the physicochemical cell properties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Proteínas de Bactérias / Aderência Bacteriana / Biofilmes / Fatores de Virulência / Listeria monocytogenes Idioma: En Revista: Food Microbiol Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Proteínas de Bactérias / Aderência Bacteriana / Biofilmes / Fatores de Virulência / Listeria monocytogenes Idioma: En Revista: Food Microbiol Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido