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Effect of soluble factors derived from ZR 75.30 breast cancer cells on endothelial activation.
Escalona-Guzman, Alma R; Hernández-Garcia, Ricardo; Vadillo-Ortega, Felipe; Lopez-Marure, Rebeca; Mejia-Rangel, Janini; Mitre-Aguilar, Irma B; Cabrera-Quintero, Alberto J; Zentella-Dehesa, Alejandro.
Afiliação
  • Escalona-Guzman AR; Biochemistry Unit, Guillermo Soberon Acevedo, Department of Genomic Medicine and Environmental Toxicology, Biomedical Research Institute (IIBO), National Autonomous University of Mexico (UNAM) Institute of Medical Sciences, Nutrition Salvador Zubiran (INCMNSZ) Mexico City, Mexico.
  • Hernández-Garcia R; Program of Breast Cancer, IIBO, UNAM Mexico City, Mexico.
  • Vadillo-Ortega F; PhD Program in Biomedical Sciences, UNAM Mexico City, Mexico.
  • Lopez-Marure R; Department of Pharmacology, Centre of Research and Advanced Studies, National Polytechnic Institute (CINVESTAV-IPN) Mexico City, Mexico.
  • Mejia-Rangel J; Liaison Unit of the Faculty of Medicine of UNAM in the National Institute of Genomic Medicine (INMEGEN) Mexico City, Mexico.
  • Mitre-Aguilar IB; Cell Biology Laboratory, Department of Physiology, National Institute of Cardiology Ignacio Chavez (INCICH) Mexico City, Mexico.
  • Cabrera-Quintero AJ; Genetics and Molecular Biology, Centre of Research and Advanced Studies, National Polytechnic Institute (CINVESTAV-IPN) Mexico City, Mexico.
  • Zentella-Dehesa A; Biochemistry Unit, Guillermo Soberon Acevedo, Department of Genomic Medicine and Environmental Toxicology, Biomedical Research Institute (IIBO), National Autonomous University of Mexico (UNAM) Institute of Medical Sciences, Nutrition Salvador Zubiran (INCMNSZ) Mexico City, Mexico.
Int J Clin Exp Pathol ; 11(2): 685-694, 2018.
Article em En | MEDLINE | ID: mdl-31938154
In this study, we analyzed soluble factors secreted by two Estrogen Receptor Positive (ER-α) human breast cancer cell lines, ZR 75.30 (luminal B) and MCF7 (luminal A), and evaluated their effect on endothelial activation. The composition of tumoral soluble factors (TSFs) was analyzed by ELISA (Bio-Plex). TSFs from ZR 75.30 cells expressed higher levels of TNF, IFN-γ, IL-6, and IL-8 compared to TSFs from MCF-7 cells. TSFs from ZR 75.30 cells induced a pro-adhesive phenotype in human umbilical vein endothelial cells (HUVECs), as characterized by increased monocytic cell adhesion, adhesion molecule expression and NF-κB activation and decreased IκB-α expression. Conversely, TSFs from MCF-7 cells exerted none of these effects on HUVECs. We then added TNF, IFN-γ, IL-6 or IL-8 alone or in combination with TSFs from MCF-7 cells to HUVECs. Only the combinations that included TNF induced endothelial activation. A neutralizing antibody against IL-1ß (this cytokine was not measured in the ELISA) had a modest blocking effect on cellular adhesion or the expression of adhesion molecules induced by TSFs from ZR 75.30 cells in HUVECs. However neutralizing antibodies against TNF, IFN-γ, IL-6 or IL-8 had no effect. Our results suggest that although TNF is an inducer of endothelial cell activation, it is not the only molecule that is responsible for this effect in TSFs from ZR 75.30 cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Clin Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Clin Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos