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Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach.
Tavares, Tássia Silva; da Rocha, Eduardo Pereira; Esteves Nogueira, Francisco Guilherme; Torres, Juliana Arriel; Silva, Maria Cristina; Kuca, Kamil; Ramalho, Teodorico C.
Afiliação
  • Tavares TS; Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, Brazil.
  • da Rocha EP; Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, Brazil.
  • Esteves Nogueira FG; Department of Chemistry Engineering, Federal University of São Carlos, 13565-905 São Carlos, SP, Brazil.
  • Torres JA; Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, Brazil.
  • Silva MC; Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, Brazil.
  • Kuca K; Department of Chemistry, Faculty of Science, University of Hradec Králové, 500 03 Hradec Králové, Czech Republic.
  • Ramalho TC; Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, Brazil.
Molecules ; 25(2)2020 Jan 08.
Article em En | MEDLINE | ID: mdl-31936386
Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Δ-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (-1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (-0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (-0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 °C, and 30 min) showed high efficiency for FA oxidation (82%).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos Férricos / Enzimas Imobilizadas / Peroxidase do Rábano Silvestre Tipo de estudo: Prognostic_studies Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos Férricos / Enzimas Imobilizadas / Peroxidase do Rábano Silvestre Tipo de estudo: Prognostic_studies Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça