Novel transglycosylation activity of ß-N-acetylglucosaminidase of Lecanicillium lecanii produced by submerged culture.
Int J Biol Macromol
; 145: 759-767, 2020 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-31887380
N-acetylglucosaminidase produced from Lecanicillium lecanii on submerged culture displayed hydrolytic and transglycosylation activities. The highest specific activity for the enzyme was 1.87 U/mg after 120 h of culture. The chromatographic purification for a single protein fraction showed a molecular weight of 50.4 kDa and hydrolytic N-acetylglucosaminidase activity of 17.59 U/mg at 37 °C and pH 6. This enzyme was able to transglycosylate and to synthesize oligosaccharides from 2 to 6 units with a degree of acetylation between 100 and 26% employing glucose, mannose, N-acetyl-D-glucosamine and N-acetyl-D-lactosamine as donor substrates. Optimal conditions of temperature and pH were determined for both types of enzymatic activities.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilglucosaminidase
/
Hypocreales
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Holanda