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Profiling epigenetic changes in human cell line induced by atrazine exposure.
Sánchez, Oscar F; Lin, Li; Bryan, Chris J; Xie, Junkai; Freeman, Jennifer L; Yuan, Chongli.
Afiliação
  • Sánchez OF; Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907, USA; Department of Nutrition and Biochemistry, Pontificia Universidad Javeriana, Bogotá, 110231, Colombia.
  • Lin L; Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
  • Bryan CJ; Department of Statistics, Purdue University, West Lafayette, IN, 47907, USA.
  • Xie J; Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
  • Freeman JL; School of Health Sciences, Purdue University, West Lafayette, IN, 47907, USA.
  • Yuan C; Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907, USA. Electronic address: cyuan@purdue.edu.
Environ Pollut ; 258: 113712, 2020 Mar.
Article em En | MEDLINE | ID: mdl-31875570
How environmental chemicals can affect and exert their toxic effect at a molecular level has gained significant interest in recent years, not only for understanding their immediate health implications over exposed individuals, but also for their subsequent progeny. Atrazine (ATZ) is a commonly used herbicide in the U.S. and a long-suspected endocrine disrupting chemical. The molecular mechanism conferring long-term adverse health outcomes, however, remain elusive. Here, we explored changes in epigenetic marks that arise after exposure to ATZ at selected doses using image-based analysis coupled with data clustering. Significant decreases in methylated CpG (meCpG) and histone 3 lysine 9 tri-methylated (H3K9me3) were observed in the selected human cell line with a clear spatial preference. Treating cells with ATZ leads to the loss of a subpopulation of cells with high meCpG levels as identified in our clustering and histogram analysis. A similar trend was observed in H3K9me3 potentially attributing to the cross-talking between meCpG and H3K9me3. Changes in meCpG are likely to be associated with alterations in epigenetic enzyme expression levels regulating meCpG and persist after the removal of ATZ source which collectively provide a plausible mechanism for long-term ATZ-induced toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atrazina / Metilação de DNA / Epigênese Genética / Herbicidas Limite: Humans Idioma: En Revista: Environ Pollut Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atrazina / Metilação de DNA / Epigênese Genética / Herbicidas Limite: Humans Idioma: En Revista: Environ Pollut Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido