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The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse.
Cintra, Lorena Cardoso; da Costa, Isabelle Cristine; de Oliveira, Izadora Cristina Moreira; Fernandes, Amanda Gregorim; Faria, Syd Pereira; Jesuíno, Rosália Santos Amorin; Ravanal, Maria Cristina; Eyzaguirre, Jaime; Ramos, Luiz Pereira; de Faria, Fabrícia Paula; Ulhoa, Cirano José.
Afiliação
  • Cintra LC; Department of Cellular Biology, University of Brasília, Brasília, Brazil; School of Veterinary and Animal Sciences, Federal University of Goiás, Goiânia, GO, Brazil.
  • da Costa IC; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • de Oliveira ICM; Department of Cellular Biology, University of Brasília, Brasília, Brazil; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • Fernandes AG; Department of Cellular Biology, University of Brasília, Brasília, Brazil; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • Faria SP; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • Jesuíno RSA; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • Ravanal MC; Department of Biological Sciences, Andrés Bello University, Santiago, Chile; Instituto de Ciencia y Tecnología de los Alimentos (ICYTAL), Facultad de Ciencias Agrarias, Universidad Austral de Chile, Valdivia, Chile.
  • Eyzaguirre J; Department of Biological Sciences, Andrés Bello University, Santiago, Chile.
  • Ramos LP; Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
  • de Faria FP; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil.
  • Ulhoa CJ; Department of Cellular Biology, University of Brasília, Brasília, Brazil; Department of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, GO, Brazil. Electronic address: cjulhoa@gmail.com.
Enzyme Microb Technol ; 133: 109447, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31874680
To increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the plant cell wall, a combination of enzymes with complementary activities is required. Xylan is the main hemicellulose component of energy crops and for its complete hydrolysis a system consisting of several enzymes acting cooperatively, including endoxylanases (XYN), ß-xylosidases (XYL) and α-l-arabinofuranosidases (ABF) is necessary. The current work aimed at evaluating the effect of recombinant hemicellulolytic enzymes on the enzymatic hydrolysis of steam-exploded sugarcane bagasse (SEB). One recombinant endoxylanase (HXYN2) and one recombinant ß-xylosidase (HXYLA) from Humicola grisea var thermoidea, together with an α-l-arabinofuranosidase (AFB3) from Penicillium pupurogenum, all produced in Pichia pastoris, were used to formulate an efficient enzyme mixture for SEB hydrolysis using a 23 Central Composite Rotatable Design (CCRD). The most potent enzyme for SEB hydrolysis was ABF3. Subsequently, the optimal enzyme mixture was used in combination with commercial cellulases (Accellerase 1500), either simultaneously or in sequential experiments. The supplementation of Accellerase 1500 with hemicellulases enhanced the glucose yield from SEB hydrolysis by 14.6%, but this effect could be raised to 50% when hemicellulases were added prior to hydrolysis with commercial cellulases. These results were supported by scanning electron microscopy, which revealed the effect of enzymatic hydrolysis on SEB fibers. Our results show the potential of complementary enzyme activities to improve enzymatic hydrolysis of SEB, thus improving the efficiency of the hydrolytic process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vapor / Celulose / Saccharum / Glicosídeo Hidrolases Idioma: En Revista: Enzyme Microb Technol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vapor / Celulose / Saccharum / Glicosídeo Hidrolases Idioma: En Revista: Enzyme Microb Technol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos