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Image mean square displacement to study the lateral mobility of Angiotensin II type 1 and Endothelin 1 type A receptors on living cells.
Planes, Nadir; Vanderheyden, Patrick P M L; Gratton, Enrico; Caballero-George, Catherina.
Afiliação
  • Planes N; Center of Innovation and Technology Transfer, Institute of Scientific Research and High Technology Services (INDICASAT AIP), Panama City, Panama.
  • Vanderheyden PPML; Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
  • Gratton E; Faculty of Sciences and Bioengineering Sciences, Free University of Brussels, Belgium.
  • Caballero-George C; Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California.
Microsc Res Tech ; 83(4): 381-392, 2020 Apr.
Article em En | MEDLINE | ID: mdl-31837080
The lateral mobility of membrane receptors provides insights into the molecular interactions of protein binding and the complex dynamic plasma membrane. The image mean square displacement (iMSD) analysis is a method used to extract qualitative and quantitative information of the protein diffusion law and infers how diffusion dynamic processes may change when the cellular environment is modified. The aim of the study was to describe the membrane diffusing properties of two G-protein-coupled receptors namely Angiotensin II type 1 (AT1 ) and Endothelin 1 type A (ETA ) receptors and their corresponding receptor-ligand complexes in living cells using total internal reflection fluorescent microscopy and iMSD analysis. This study showed that both AT1 and ETA receptors displayed a mix of three modes of diffusion: free, confined, and partially confined. The confined mode was the predominant at the plasma membrane of living cells and was not affected by ligand binding. However, the local diffusivity and the confinement zone of AT1 receptors were reduced by the binding of its antagonist losartan, and the long-range diffusion with the local diffusivity coefficient of ETA receptors was reduced upon exposure to its antagonist BQ123. To the best of our knowledge, this is the first study addressing the protein diffusion laws of these two receptors on living cells using total internal reflection fluorescence microscopy and iMSD.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Receptor Tipo 1 de Angiotensina / Receptor de Endotelina A / Microscopia de Fluorescência Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: Microsc Res Tech Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Panamá País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Processamento de Imagem Assistida por Computador / Receptor Tipo 1 de Angiotensina / Receptor de Endotelina A / Microscopia de Fluorescência Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: Microsc Res Tech Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Panamá País de publicação: Estados Unidos