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Exosomes secreted from miRNA-29b-modified mesenchymal stem cells repaired spinal cord injury in rats.
Yu, Tao; Zhao, Cunju; Hou, Shouzhi; Zhou, Weijie; Wang, Baoxin; Chen, Yunzhen.
Afiliação
  • Yu T; Department of Spinal Surgery, Qilu Hospitial of ShanDong University, Jinan, Shandong, China.
  • Zhao C; Department of Orthopedics, Liaocheng People's Hospitial, Liaocheng, Shandong, China.
  • Hou S; Department of Spinal Surgery, Qilu Hospitial of ShanDong University, Jinan, Shandong, China.
  • Zhou W; Department of Orthopedics, Liaocheng People's Hospitial, Liaocheng, Shandong, China.
  • Wang B; Department of Radiology, Liaocheng People's Hospitial, Liaocheng, Shandong, China.
  • Chen Y; Department of Orthopedics, Liaocheng People's Hospitial, Liaocheng, Shandong, China.
Braz J Med Biol Res ; 52(12): e8735, 2019.
Article em En | MEDLINE | ID: mdl-31826179
Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected intravenously with exosomes (control exosomes, miRNA-29b exosomes) and BMSCs (miR NC, miRNA-29b) through the tail vein. The expression of miRNA-29b in spinal cord tissues of SCI rats was detected by qRT-PCR. The hind limb motor function was evaluated by Basso Beattie Bresnahan (BBB) score. The histopathological damage and neuronal regeneration in spinal cord tissues was observed by HE staining and immunohistochemistry, respectively. The injection of miRNA-29b exosomes and miRNA-29b BMSCs both significantly increased the expression of miRNA-29b in spinal cord tissues of SCI rats (P<0.05). Compared with SCI rats, rats in the miRNA-29b exosomes and the miRNA-29b groups exhibited improved SCI, including increased BBB score, NF200 and GAP-43 positive neurons, as well as decreased contractile nerve cell numbers and GFAP positive neurons (all P<0.05). The relieving degree of SCI was significantly higher in the miRNA-29b exosomes group than in the miRNA-29b BMSCs group (P<0.05). Exosomes secreted from miRNA-29b-modified BMSCs were effective in the repair of SCI in rats.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Transfecção / Recuperação de Função Fisiológica / MicroRNAs / Transplante de Células-Tronco Mesenquimais / Exossomos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Transfecção / Recuperação de Função Fisiológica / MicroRNAs / Transplante de Células-Tronco Mesenquimais / Exossomos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Brasil