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Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba.
Jerez Puebla, Luis Enrique; Núñez Fernández, Fidel A; Fraga, Jorge; Rivero, Lázara Rojas; Millán, Iraís Atencio; Valdés, Lucía Ayllón; Silva, Isabel Martínez; Müller, Norbert; Robertson, Lucy J.
Afiliação
  • Jerez Puebla LE; Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6(1/2) e/Autopista Nacional y Carretera, Central, La Habana, Cuba.
  • Núñez Fernández FA; Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6(1/2) e/Autopista Nacional y Carretera, Central, La Habana, Cuba.
  • Fraga J; Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6(1/2) e/Autopista Nacional y Carretera, Central, La Habana, Cuba.
  • Rivero LR; Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6(1/2) e/Autopista Nacional y Carretera, Central, La Habana, Cuba.
  • Millán IA; Department of Parasitology, Tropical Medicine Institute "Pedro Kourí", Autopista Novia del Mediodía Km 6(1/2) e/Autopista Nacional y Carretera, Central, La Habana, Cuba.
  • Valdés LA; Paedriatic Hospital "William Soler", Avenida 100 y Perla Altahabana, Ciudad de La Habana, Cuba.
  • Silva IM; Paedriatic Hospital "William Soler", Avenida 100 y Perla Altahabana, Ciudad de La Habana, Cuba.
  • Müller N; Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Robertson LJ; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: lucy.robertson@nmbu.no.
Exp Parasitol ; 209: 107814, 2020 Feb.
Article em En | MEDLINE | ID: mdl-31816280
Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana, Cuba between 2010 and 2013, with the aim of comparing the results from molecular (PCR) approaches targeting different genes in order to assign with confidence 224 isolates of G. duodenalis to the correct assemblages. In each sub-study, following DNA isolation by the phenol/chloroform/isoamyl alcohol extraction method, PCR targeting the triose phosphate isomerase (tpi) gene was used for molecular characterization, as well as one additional PCR-method targeting another gene or pair of genes. DNA amplification was obtained in 87%, 83%, and 80% in the three sub-studies. Although excellent agreement (kappa index = 1) was recorded between results from some pairs of genes, for other combinations only moderate or substantial agreement was achieved. These results highlight the importance of interpretation of genotyping data, especially when single genetic markers are used. From the results of our studies, PCR targeting a combination of the tpi gene and the intergenic spacer region of rDNA may be a useful approach for the molecular characterization of G. duodenalis isolates.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Giardíase / Giardia lamblia / Técnicas de Genotipagem Tipo de estudo: Observational_studies Limite: Animals / Child / Child, preschool / Humans País/Região como assunto: Caribe / Cuba Idioma: En Revista: Exp Parasitol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Cuba País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Giardíase / Giardia lamblia / Técnicas de Genotipagem Tipo de estudo: Observational_studies Limite: Animals / Child / Child, preschool / Humans País/Região como assunto: Caribe / Cuba Idioma: En Revista: Exp Parasitol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Cuba País de publicação: Estados Unidos