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KRAS mutations in implant-associated peripheral giant cell granuloma.
Martins-Chaves, Roberta Rayra; Guimarães, Letícia Martins; Pereira, Thaís Dos Santos Fontes; Pereira, Núbia Braga; Chrcanovic, Bruno Ramos; Fonseca, Felipe Paiva; Lafuente-Ibáñez de Mendoza, Irene; Aguirre-Urizar, José Manuel; Gomes, Carolina Cavaliéri; Gomez, Ricardo Santiago.
Afiliação
  • Martins-Chaves RR; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Guimarães LM; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Pereira TDSF; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Pereira NB; Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Chrcanovic BR; Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden.
  • Fonseca FP; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Lafuente-Ibáñez de Mendoza I; Unit of Oral and Maxillofacial Pathology of the Dental Clinic Service, Department of Stomatology II, University of the Basque Country (UPV-EHU), Bizkaia, Spain.
  • Aguirre-Urizar JM; Unit of Oral and Maxillofacial Pathology of the Dental Clinic Service, Department of Stomatology II, University of the Basque Country (UPV-EHU), Bizkaia, Spain.
  • Gomes CC; Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
  • Gomez RS; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Oral Dis ; 26(2): 334-340, 2020 Mar.
Article em En | MEDLINE | ID: mdl-31758745
OBJECTIVES: To investigate the molecular pathogenesis of implant-associated peripheral giant cell granuloma (IA-PGCG). METHODS: A convenience sample of 15 IA-PGCG cases was selected. Hotspot mutations of KRAS, FGFR1, and TRPV4 genes, previously reported in conventional giant cell lesions of the jaws, were investigated by Sanger sequencing. As these mutations could activate MAPK/ERK pathway, the expression of phospho-ERK1/2 was also evaluated by immunohistochemistry. RESULTS: KRAS mutations were detected in 8/15 (53.4%) samples. Similar to conventional peripheral giant cell granuloma, the KRAS mutations most frequently occurred in codon 146 (p.A146V, n = 3), followed by codon 12 (p.G12A and p.G12D, n = 1 each) and codon 14 (p.V14L, n = 1). Variants of unknown significance (VUS) were also detected in two cases, affecting codons 37 (p.E37K) and 127 (p.T127I). All samples showed wild-type (WT) sequences for FGFR1 and TRPV4 genes. Consistent with MAPK/ERK pathway activation, all mononuclear cells of the lesion showed strong staining for phospho-ERK1/2 protein in the immunohistochemical analysis. CONCLUSIONS: KRAS mutations and activation of the MAPK-ERK signaling pathway occur in IA-PGCG. This is the first study to demonstrate cancer-associated gene mutations in a non-neoplastic reactive condition associated with dental implants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Granuloma de Células Gigantes / Implantes Dentários / Proteínas Proto-Oncogênicas p21(ras) Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Oral Dis Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Dinamarca
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Granuloma de Células Gigantes / Implantes Dentários / Proteínas Proto-Oncogênicas p21(ras) Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Oral Dis Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Dinamarca