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Virus isolation and genotype identification of human respiratory syncytial virus in Guizhou Province, China.
Zhao, Ting; Ye, Zhixu; Wang, Binlin; Cui, Yuxia; Nie, Yingjie; Yang, Bin; Chen, Kun; Zhang, Hua; Hu, Fangfang; Yu, Fuxun.
Afiliação
  • Zhao T; Guizhou University, Medical College, Guiyang, China; Guizhou University, Guizhou Provincial People's Hospital, Department of Central Laboratory, GuiyangChina. Electronic address: 1577065205@qq.com.
  • Ye Z; Guizhou University, Guizhou Provincial People's Hospital, Department of Pediatrics, Guiyang, China; Guizhou Provincial People's Hospital, NHC Key Laboratory of Pulmonary Immunological Diseases, Guiyang, China.
  • Wang B; People's Hospital of QianNan, Department of Clinical Labotatory, Guizhou Province, China.
  • Cui Y; Guizhou University, Guizhou Provincial People's Hospital, Department of Pediatrics, Guiyang, China.
  • Nie Y; Guizhou University, Guizhou Provincial People's Hospital, Department of Central Laboratory, GuiyangChina; Guizhou Provincial People's Hospital, NHC Key Laboratory of Pulmonary Immunological Diseases, Guiyang, China.
  • Yang B; Guizhou University, Guizhou Provincial People's Hospital, Department of Central Laboratory, GuiyangChina; Guizhou Provincial People's Hospital, NHC Key Laboratory of Pulmonary Immunological Diseases, Guiyang, China.
  • Chen K; Guizhou University, Guizhou Provincial People's Hospital, Department of Central Laboratory, GuiyangChina; Guizhou Provincial People's Hospital, NHC Key Laboratory of Pulmonary Immunological Diseases, Guiyang, China.
  • Zhang H; Guizhou University, Guizhou Provincial People's Hospital, Department of Clinical Laboratory, Guiyang, China.
  • Hu F; Guizhou University, Guizhou Provincial People's Hospital, Department of Clinical Laboratory, Guiyang, China.
  • Yu F; Guizhou University, Guizhou Provincial People's Hospital, Department of Central Laboratory, GuiyangChina; Guizhou Provincial People's Hospital, NHC Key Laboratory of Pulmonary Immunological Diseases, Guiyang, China.
Braz J Infect Dis ; 23(6): 427-434, 2019.
Article em En | MEDLINE | ID: mdl-31734172
To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence analysis showed that subgroup A was dominant. Seventy-nine percent of the isolates belonged to subgroup A, ON1 genotype, and 21 % belonged to subgroup B, BA9 genotype, which indicates that the dominant HRSV circulating in Guizhou Province was subgroup A, genotype ON1, co-circulating with a less prevalent subgroup B, genotype BA9.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Vírus Sincicial Respiratório Humano / Infecções por Vírus Respiratório Sincicial Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Child, preschool / Humans País/Região como assunto: Asia Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Vírus Sincicial Respiratório Humano / Infecções por Vírus Respiratório Sincicial Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Child, preschool / Humans País/Região como assunto: Asia Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil