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Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity.
Seribelli, Amanda Ap; Gonzales, Júlia C; de Almeida, Fernanda; Benevides, Leandro; Cazentini Medeiros, Marta I; Dos Prazeres Rodrigues, Dália; de C Soares, Siomar; Allard, Marc W; Falcão, Juliana P.
Afiliação
  • Seribelli AA; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Av. do Café, s/n°-Campus Universitário USP, Ribeirão Preto, SP, 14040-903, Brazil. jufalcao@fcfrp.usp.br.
  • Gonzales JC; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Av. do Café, s/n°-Campus Universitário USP, Ribeirão Preto, SP, 14040-903, Brazil.
  • de Almeida F; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Av. do Café, s/n°-Campus Universitário USP, Ribeirão Preto, SP, 14040-903, Brazil.
  • Benevides L; National Laboratory of Scientific Computation - LNCC, Petrópolis, Brazil.
  • Cazentini Medeiros MI; Instituto Adolfo Lutz de Ribeirão Preto - IAL, São Paulo, Brazil.
  • Dos Prazeres Rodrigues D; Fundação Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, Brazil.
  • de C Soares S; Universidade Federal do Triângulo Mineiro - UFTM, Uberaba, MG, Brazil.
  • Allard MW; Food and Drug Administration - FDA, College Park, MA, USA.
  • Falcão JP; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo - USP, Av. do Café, s/n°-Campus Universitário USP, Ribeirão Preto, SP, 14040-903, Brazil.
Braz J Microbiol ; 51(1): 53-64, 2020 Mar.
Article em En | MEDLINE | ID: mdl-31728978
Salmonella Typhimurium sequence type 313 (S. Typhimurium ST313) has caused invasive disease mainly in sub-Saharan Africa. In Brazil, ST313 strains have been recently described, and there is a lack of studies that assessed by whole genome sequencing (WGS)-the relationship of these strains. The aims of this work were to study the phylogenetic relationship of 70 S. Typhimurium genomes comparing strains of ST313 (n = 9) isolated from humans and food in Brazil among themselves, with other STs isolated in this country (n = 31) and in other parts of the globe (n = 30) by 16S rRNA sequences, the Gegenees software, whole genome multilocus sequence typing (wgMLST), and average nucleotide identity (ANI) for the genomes of ST313. Additionally, pangenome analysis was performed to verify the heterogeneity of these genomes. The phylogenetic analyses showed that the ST313 genomes were very similar among themselves. However, the ST313 genomes were usually clustered more distantly to other STs of strains isolated in Brazil and in other parts of the world. By pangenome calculation, the core genome was 2,880 CDSs and 4,171 CDSs singletons for all the 70 S. Typhimurium genomes studied. Considering the 10 ST313 genomes analyzed the core genome was 4,112 CDSs and 76 CDSs singletons. In conclusion, the ST313 genomes from Brazil showed a high similarity among them which information might eventually help in the development of vaccines and antibiotics. The pangenome analysis showed that the S. Typhimurium genomes studied presented an open pangenome, but specifically tending to become close for the ST313 strains.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Salmonella typhimurium / Genoma Bacteriano / Microbiologia de Alimentos Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Salmonella typhimurium / Genoma Bacteriano / Microbiologia de Alimentos Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil