Your browser doesn't support javascript.
loading
Annona coriacea Mart. Fractions Promote Cell Cycle Arrest and Inhibit Autophagic Flux in Human Cervical Cancer Cell Lines.
Gomes, Izabela N Faria; Silva-Oliveira, Renato J; Oliveira Silva, Viviane A; Rosa, Marcela N; Vital, Patrik S; Barbosa, Maria Cristina S; Dos Santos, Fábio Vieira; Junqueira, João Gabriel M; Severino, Vanessa G P; Oliveira, Bruno G; Romão, Wanderson; Reis, Rui Manuel; Ribeiro, Rosy Iara Maciel de Azambuja.
Afiliação
  • Gomes INF; Experimental Pathology Laboratory, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. izabela.faria.tk@hotmail.com.
  • Silva-Oliveira RJ; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. izabela.faria.tk@hotmail.com.
  • Oliveira Silva VA; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. renatokjso@gmail.com.
  • Rosa MN; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. vivianeaos@gmail.com.
  • Vital PS; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. nr.marcela@gmail.com.
  • Barbosa MCS; Experimental Pathology Laboratory, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. patrikdasilvavital@gmail.com.
  • Dos Santos FV; Laboratory of Cell Biology and Mutagenesis, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. mariacristina@ufsj.edu.br.
  • Junqueira JGM; Laboratory of Cell Biology and Mutagenesis, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. fabiosantos@ufsj.edu.br.
  • Severino VGP; Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil. jgmjunqueira@gmail.com.
  • Oliveira BG; Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil. vanessa.pasqualotto@gmail.com.
  • Romão W; Petroleomic and forensic chemistry laboratory, Department of Chemistry, Federal Institute of Espirito Santo, Vitória, ES 29075-910, Brazil. brunoliveir_ra20@msn.com.
  • Reis RM; Petroleomic and forensic chemistry laboratory, Department of Chemistry, Federal Institute of Espirito Santo, Vitória, ES 29075-910, Brazil. wandersonromao@gmail.com.
  • Ribeiro RIMA; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. ruireis.hcb@gmail.com.
Molecules ; 24(21)2019 Nov 01.
Article em En | MEDLINE | ID: mdl-31683835
Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Neoplasias do Colo do Útero / Annona / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Neoplasias do Colo do Útero / Annona / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça