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Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation.
Ramos, Yassel; González, Annia; Sosa-Acosta, Patricia; Perez-Riverol, Yasset; García, Yairet; Castellanos-Serra, Lila; Gil, Jeovanis; Sánchez, Aniel; González, Luis J; Besada, Vladimir.
Afiliação
  • Ramos Y; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • González A; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • Sosa-Acosta P; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • Perez-Riverol Y; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
  • García Y; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • Castellanos-Serra L; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • Gil J; Clinical Protein Science & Imaging, Biomedical Centre, Department of Biomedical Engineering, Lund University, Lund, Lund, Sweden.
  • Sánchez A; Section for Clinical Chemistry, Department of Translational Medicine, Lund University, Skåne University Hospital Malmö, Malmö, Sweden.
  • González LJ; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
  • Besada V; Department of Proteomics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba.
J Sep Sci ; 42(24): 3712-3717, 2019 Dec.
Article em En | MEDLINE | ID: mdl-31625274
Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Dodecilsulfato de Sódio / Proteômica Idioma: En Revista: J Sep Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Dodecilsulfato de Sódio / Proteômica Idioma: En Revista: J Sep Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha