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Protein-coated dsDNA nanostars with high structural rigidity and high enzymatic and thermal stability.
Sanchez-Rueda, Eddie G; Rodriguez-Cristobal, Estefani; Moctezuma González, Claudia L; Hernandez-Garcia, Armando.
Afiliação
  • Sanchez-Rueda EG; Laboratory of Biomolecular Engineering and Bionanotechnology, Chemistry of Biomacromolecules Department, Institute of Chemistry, National Autonomous University of Mexico, Ciudad Universitaria, Coyoacan, Mexico City 04310, Mexico. armandohg@iquimica.unam.mx.
  • Rodriguez-Cristobal E; Laboratory of Biomolecular Engineering and Bionanotechnology, Chemistry of Biomacromolecules Department, Institute of Chemistry, National Autonomous University of Mexico, Ciudad Universitaria, Coyoacan, Mexico City 04310, Mexico. armandohg@iquimica.unam.mx.
  • Moctezuma González CL; Laboratory of Biomolecular Engineering and Bionanotechnology, Chemistry of Biomacromolecules Department, Institute of Chemistry, National Autonomous University of Mexico, Ciudad Universitaria, Coyoacan, Mexico City 04310, Mexico. armandohg@iquimica.unam.mx.
  • Hernandez-Garcia A; Laboratory of Biomolecular Engineering and Bionanotechnology, Chemistry of Biomacromolecules Department, Institute of Chemistry, National Autonomous University of Mexico, Ciudad Universitaria, Coyoacan, Mexico City 04310, Mexico. armandohg@iquimica.unam.mx.
Nanoscale ; 11(40): 18604-18611, 2019 Oct 28.
Article em En | MEDLINE | ID: mdl-31578534
DNA nanotechnology creates precise shape-specific nanostructures through the self-assembly of short ssDNA oligonucleotides. One such shape, which has relevant biomedical applications due to its multivalency, is the star. However, building star-like nanostructures with a large size (>100 nm) using ssDNA is complex and challenging. This study presents a novel strategy to prepare stiff and large dsDNA nanostars by assembling duplex DNA fragments into star-shapes that are subsequently coated with a virus-inspired protein. The protein binds dsDNA and overcomes the high structural flexibility of naked dsDNA. The nanostar-like dsDNA templates with up to six arms were prepared by self-assembly of PCR-produced dsDNA fragments (211 to 722 bp) with a central DNA junction. Through gel electrophoresis and Atomic Force Microscopy it is demonstrated that single dsDNA nanostars are self-assembled and coated with the protein, and this has a large stiffening effect on the nanostar. Furthermore, the coating significantly enhances stability at high temperatures and protects nanostars against nuclease degradation for at least 10 hours. This study shows that DNA-binding proteins can be harnessed as structural "rigidifiers" of flexible branched dsDNA templates. This strategy opens a way to prepare structurally defined hybrid protein-dsDNA nanostructures that could be exploited as building blocks for novel DNA nanomaterials.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Estreptavidina / Nanoestruturas / Temperatura Alta / Conformação de Ácido Nucleico Idioma: En Revista: Nanoscale Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Estreptavidina / Nanoestruturas / Temperatura Alta / Conformação de Ácido Nucleico Idioma: En Revista: Nanoscale Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido