Glycine receptor is differentially expressed in the rat retina at early stages of streptozotocin-induced diabetes.
Neurosci Lett
; 712: 134506, 2019 11 01.
Article
em En
| MEDLINE
| ID: mdl-31541724
Diabetes mellitus is a metabolic disease that leads to several complications which include retinopathy. Neuronal abnormalities have been reported to appear before microvasculature alterations. We analyzed the expression levels of GlyR subunits in the retina at 7, 20, and 45 days after streptozotocin-induced diabetes to gain insight into the pathogenesis of diabetic retinopathy. We determined the mRNA and protein expression by qPCR and western blot, respectively. The mRNA and protein expression of the α1 subunit was not altered over the study period; however, they were slightly reduced in α2 yet statistically significant. While protein expression of α3 subunit was only reduced at 45 days diabetes. The mRNA and protein expression of the α4 subunit was remarkably decreased since day 7 of diabetes, remaining only â¼20% on day 45 of diabetes. Surprisingly, the mRNA of the ß subunit was highly increased, while its protein levels were not changed. The decrease in GlyR α subunits expression in the retina from diabetic animals suggest a perturbation in the inhibitory glycine signaling pathway, which might be related to the visual alterations observed in diabetes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Receptores de Glicina
/
Diabetes Mellitus Experimental
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Irlanda