Your browser doesn't support javascript.
loading
Luciferase gene of a Caribbean fireworm (Syllidae) from Puerto Rico.
Mitani, Yasuo; Yasuno, Rie; Futahashi, Ryo; Oakley, Todd H; Ohmiya, Yoshihiro.
Afiliação
  • Mitani Y; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan. mitani-y@aist.go.jp.
  • Yasuno R; Biomedical Research Institute, AIST, Tsukuba, 305-8566, Japan.
  • Futahashi R; Bioproduction Research Institute, AIST, Tsukuba, 305-8566, Japan.
  • Oakley TH; Marine Science Institute, University of California, Santa Barbara, CA, 93106, USA.
  • Ohmiya Y; Biomedical Research Institute, AIST, Tsukuba, 305-8566, Japan.
Sci Rep ; 9(1): 13015, 2019 09 10.
Article em En | MEDLINE | ID: mdl-31506523
The fireworms Odontosyllis spp. are globally distributed and well-known for their characteristic and fascinating mating behavior, with secreted mucus emitting bluish-green light. However, knowledge about the molecules involved in the light emission are still scarce. The fireworms are believed to emit light with a luciferin-luciferase reaction, but biochemical evidence of the luciferase is established for only one species living in Japan and no information is available for its luciferin structure. In this study, we identified a luciferase gene from a related Puerto Rican fireworm. We identified eight luciferase-like genes in this Puerto Rican fireworm, finding amino acid identities between Japanese and Puerto Rican luciferase-like genes to be less than 60%. We confirmed cross reactivity of extracts of the Japanese fireworm luciferin with a recombinant Puerto Rican luciferase (PR1). The emission spectrum of recombinant PR1 was similar to the crude extract of the native luciferase, suggesting that PR1 is a functional luciferase of this Puerto Rican fireworm. Our results indicate that the molecular mechanism of luminescence is widely conserved among fireworms.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliquetos / Proteínas Recombinantes / Luminescência / Luciferases Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: Asia / Caribe / Puerto rico Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliquetos / Proteínas Recombinantes / Luminescência / Luciferases Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: Asia / Caribe / Puerto rico Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido