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Araucaria angustifolia (Bertol.) Kuntze has neuroprotective action through mitochondrial modulation in dopaminergic SH-SY5Y cells.
Branco, Catia Santos; Duong, Angela; Machado, Alencar Kolinski; Wu, Abbie; Scola, Gustavo; Andreazza, Ana Cristina; Salvador, Mirian.
Afiliação
  • Branco CS; Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, 95070 560, Brazil. csbranc1@ucs.br.
  • Duong A; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
  • Machado AK; Franciscan University, Santa Maria, RS, 97010032, Brazil.
  • Wu A; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
  • Scola G; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
  • Andreazza AC; Department of Psychiatry, University of Toronto, Toronto, ON, Canada.
  • Salvador M; Centre for Addiction and Mental Health - CAMH, Toronto, ON, Canada.
Mol Biol Rep ; 46(6): 6013-6025, 2019 Dec.
Article em En | MEDLINE | ID: mdl-31452047
Brain disorders (BD) including neuropsychiatric and neurodegenerative diseases, are often associated with impairments in mitochondrial function and oxidative damage that can lead to neuronal injury. The mitochondrial complex I enzyme is one of the main sites of ROS generation and is implicated in many BD pathophysiologies. Despite advances in therapeutics for BD management, conventional pharmacotherapy still cannot efficiently control neuronal redox imbalance and mitochondrial dysfunction. Araucaria angustifolia is one of the main pine species in South America and presents a notable therapeutic history in folk medicine. A. angustifolia extract (AAE), obtained from the natural waste named bracts, is rich in flavonoids; molecules able to regulate cell redox metabolism. We examined the effects of AAE on rotenone-induced mitochondrial complex I dysfunction in human dopaminergic SH-SY5Y cells. AAE restored complex I assembly and activity mainly through overexpression of NDUFS7 protein and NDUFV2 gene levels. These findings were accompanied by a reduction in the generation of neuronal reactive oxygen species and lipid peroxidation. Our data demonstrates, for the first time, that AAE exerts in vitro neuroprotective effects, thus making it an interesting source for future drug development in BD-associated mitochondrial dysfunctions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Extratos Vegetais / Complexo I de Transporte de Elétrons / Araucaria Limite: Humans País/Região como assunto: America do sul Idioma: En Revista: Mol Biol Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Extratos Vegetais / Complexo I de Transporte de Elétrons / Araucaria Limite: Humans País/Região como assunto: America do sul Idioma: En Revista: Mol Biol Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda