Cell volume regulation in the trypanosomatid Herpetomonas samuelpessoai.
Braz J Med Biol Res
; 21(2): 379-84, 1988.
Article
em En
| MEDLINE
| ID: mdl-3144405
Herpetomonas samuelpessoai was submitted to hypo- and hyper-osmotic shocks in buffer and culture medium. Cell volume was evaluated with an electronic particle counter and by the transmission of a parasite suspension measured at 540 nm with a spectrophotometer. Cell volume changes after osmotic shock were transient, parasites being able to completely restore cell volume to the original values in culture medium but not in buffer. The kinetics of transmittance changes closely resembled that of volume variations. The volume regulatory increase (but not decrease) was dependent on extracellular K concentration and strongly influenced by the parasite growth phase. Evidence is presented supporting the idea that changes in transmission values can be linearly correlated to mean cell volume variations in free-floating cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Equilíbrio Hidroeletrolítico
/
Contagem de Células
/
Trypanosomatina
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil