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Polymorphism eNOS Glu298Asp modulates the inflammatory response of human peripheral blood mononuclear cells.
Maurer, Patrícia; Barbisan, Fernanda; Azzolin, Veronica Farina; Berro, Lyana Feijoo; Montagner, Renata; Duarte, Marta Maria Medeiros Frescura; da Cruz, Ivana Beatrice Mânica; Manfredini, Vanusa; Piccoli, Jacqueline da Costa Escobar.
Afiliação
  • Maurer P; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Barbisan F; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Azzolin VF; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Berro LF; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Montagner R; Postgraduate Program of Pharmaceutical Sciences, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Duarte MMMF; Lutheran University of Brazil, Santa Maria, RS, Brazil.
  • da Cruz IBM; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Manfredini V; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Piccoli JDCE; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil. Electronic address: jacquelinepiccoli@unipampa.edu.br.
Cytokine ; 125: 154812, 2020 01.
Article em En | MEDLINE | ID: mdl-31442681
INTRODUCTION: Nitric oxide is a gaseous radical produced by the nitric oxide endothelial synthase (eNOS) whose most studied physiological action is the vasodilation. However, it also acts in the defense of the organism through the formation of cytotoxic radicals, which can potentiate the inflammatory lesion of the cells. The Glu298Asp is a single nucleotide polymorphism (SNP) in the eNOS gene related to the risk of cardiovascular disease. Blacks present a higher prevalence of hypertension and cardiovascular mortality. Then, we aimed to evaluate the influence of Glu298Asp polymorphism on inflammatory response in vitro and gene expression in blacks. MATERIAL AND METHODS: Peripheral blood mononuclear cells (PBMC) from blacks with different Glu298Asp genotypes were treated with phytohemagglutinin (PHA), a mitogen and activator of T cells. Oxidative, inflammatory markers, and expression of inflammation genes were evaluated. RESULTS: The genotype frequencies were TT 6.7%; TG 29.3% and GG 64.0%. Activation of PBMCs with 125 µg of PHA modulated the expression of inflammatory genes and increased levels of inflammatory cytokines. The T allele showed increased susceptibility to inflammation (higher levels of interleukin 1, interleukin 6 and tumor necrosis factor alpha; p < 0.001). The G allele exhibited protection through higher levels of nitric oxide (p < 0.001) and fewer inflammatory cytokines. CONCLUSION: Despite methodological limitations related to in vitro assays, the whole of results suggested that Glu298Asp modulates inflammatory genes, the T allele is more susceptible to inflammation and the G allele is protective.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Citocinas / Óxido Nítrico Sintase Tipo III Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Citocinas / Óxido Nítrico Sintase Tipo III Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido