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New genus-specific primers for PCR identification of Rubrobacter strains.
Castro, Jean Franco; Nouioui, Imen; Asenjo, Juan A; Andrews, Barbara; Bull, Alan T; Goodfellow, Michael.
Afiliação
  • Castro JF; School of Natural and Environmental Sciences, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
  • Nouioui I; Department of Chemical Engineering, Biotechnology and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Beauchef 851, Santiago, Chile.
  • Asenjo JA; School of Natural and Environmental Sciences, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
  • Andrews B; Department of Chemical Engineering, Biotechnology and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Beauchef 851, Santiago, Chile.
  • Bull AT; Department of Chemical Engineering, Biotechnology and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Beauchef 851, Santiago, Chile.
  • Goodfellow M; School of Biosciences, University of Kent, Canterbury, Kent, CT2 1NJ, UK.
Antonie Van Leeuwenhoek ; 112(12): 1863-1874, 2019 Dec.
Article em En | MEDLINE | ID: mdl-31407134
A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Actinobacteria / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Evaluation_studies País/Região como assunto: America do sul Idioma: En Revista: Antonie Van Leeuwenhoek Ano de publicação: 2019 Tipo de documento: Article País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Actinobacteria / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Evaluation_studies País/Região como assunto: America do sul Idioma: En Revista: Antonie Van Leeuwenhoek Ano de publicação: 2019 Tipo de documento: Article País de publicação: Holanda