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Leishmanicidal and antimicrobial activity of primin and primin-containing extracts from Miconia willdenowii.
Viegas, Flávia Pereira Dias; Espuri, Patrícia Ferreira; Oliver, Josidel Conceição; Silva, Naiara Chaves; Dias, Amanda Latércia Tranches; Marques, Marcos José; Soares, Marisi Gomes.
Afiliação
  • Viegas FPD; Laboratory of Phytochemistry and Medicinal Chemistry, Institute of Chemistry, Federal University of Alfenas, Alfenas, Minas Gerais 37130-001, Brazil. Electronic address: fpdviegas@gmail.com.
  • Espuri PF; Laboratory of Parasitology, Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Minas Gerais, 37130-001, Brazil.
  • Oliver JC; Laboratory of Microbiology and Immunology, Institute of Biomedical Sciences, Federal University of Alfenas, 37130-001 Alfenas, Minas Gerais, Brazil.
  • Silva NC; Laboratory of Microbiology and Immunology, Institute of Biomedical Sciences, Federal University of Alfenas, 37130-001 Alfenas, Minas Gerais, Brazil.
  • Dias ALT; Laboratory of Microbiology and Immunology, Institute of Biomedical Sciences, Federal University of Alfenas, 37130-001 Alfenas, Minas Gerais, Brazil.
  • Marques MJ; Laboratory of Parasitology, Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Minas Gerais, 37130-001, Brazil. Electronic address: marques.prppg@gmail.com.
  • Soares MG; Laboratory of Phytochemistry and Medicinal Chemistry, Institute of Chemistry, Federal University of Alfenas, Alfenas, Minas Gerais 37130-001, Brazil. Electronic address: marisi.gs@gmail.com.
Fitoterapia ; 138: 104297, 2019 Oct.
Article em En | MEDLINE | ID: mdl-31404617
As a part of an ongoing bioprospective project, searching for potential medicinal plants from the Brazilian Atlantic Forest, Miconia willdenowii was selected for its potential leishmanicidal and antimicrobial activities. The crude ethanolic extract of M. willdenowii showed an inhibition of 99.7% of the promastigote forms of Leishmania amazonensis at the concentration of 80 µg/mL. Further investigation of its antimicrobial activity against pathogenic fungi and Gram positive and negative bacteria, revealed a significant antimicrobial activity. A bioguided study with its liquid-liquid partition fractions revealed the hexane fraction (Hex) as the most active against Leishmania, inhibiting 99.2% and 46.9% of the protozoan at concentrations of 40 and 20 µg/mL, respectively. Hex also showed significant antimicrobial activity against Staphylococcus aureus and Candida krusei with IC50 of 15.6 and 62.5 µg/mL, respectively. Purification of Hex led to the isolation of 2-methoxy-6-pentyl-benzoquinone (1, also known as primin) as the active metabolite, probably responsible for the observed antimicrobial and anti-leishmania effects. Primin (1) disclosed leishmanicidal activity (IC50 = 1.25 µM), showing higher potency than the standard drug amphotericin B (IC50 = 5.08 µM), with additional antifungal effects against all tested fungi species. Compound 1 also showed significant activity against S. aureus (IC50 = 8.94 µM), showing a comparable potency with the reference drug chloramphenicol (IC50 = 6.19 µM), but with a potential cytotoxicity towards peripheral human blood mononuclear cells (CC50 = 255.15 µM). Here in, the antimicrobial and anti-L. amazonensis effects of M. willdenowii are reported for the first time, as well as Primin (1) as its probable bioactive metabolite.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Benzoquinonas / Melastomataceae / Antibacterianos / Antiprotozoários Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Fitoterapia Ano de publicação: 2019 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Benzoquinonas / Melastomataceae / Antibacterianos / Antiprotozoários Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Fitoterapia Ano de publicação: 2019 Tipo de documento: Article País de publicação: Holanda