Exploring the exocellular fungal biopolymer botryosphaeran for laccase-biosensor architecture and application to determine dopamine and spironolactone.
Talanta
; 204: 475-483, 2019 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-31357322
Laccase was immobilized on a glassy carbon electrode layered with multi-walled carbon nanotubes using a film of botryosphaeran, a fungal (1â¯ââ¯3)(1â¯ââ¯6)-ß-D-glucan. This novel biosensing platform was characterized by electrochemical impedance spectroscopy and scanning electron microscopy, and applied for the determination of dopamine. Experimental variables such as enzyme concentration, pH value and operational parameters of the electroanalytical technique were optimized. Using square-wave voltammetry, there was a linear dependence of peak current and dopamine concentration within the range of 2.99-38.5⯵molâ¯L-1 with a limit of detection of 0.127⯵molâ¯L-1. The biosensor was successfully applied in the determination of dopamine in pharmaceutical injection and synthetic biological samples, and presented good selectivity even in the presence of uric acid and ascorbic acid, as well as other phenolic compounds. The different aspects regarding the operational stability of the laccase biosensor were evaluated, demonstrating good intra-day and inter-day repeatability, and long-storage stability. Furthermore, this biosensor was evaluated in the indirect determination of spironolactone by using the analytical signal of dopamine, presenting a limit of detection of 0.94⯵molâ¯L-1. The results obtained in the analysis of spironolactone in commercial pharmaceutical samples were satisfactory.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espironolactona
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Técnicas Biossensoriais
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Dopamina
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Lacase
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Glucanos
Idioma:
En
Revista:
Talanta
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda