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The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice.
Morais Mewes, Juliana; Rodrigues Silva Gasparin, Fabiana; Yoshida, Tiago; Amâncio Daniel da Silva, Mariana; Raquel Marçal Natali, Maria; Francisco Veiga Bizerra, Paulo; Sayuri Utsunomiya, Karina; Hideo Gilglioni, Eduardo; Shigueaki Mito, Marcio; Cristiane Mantovanelli, Gislaine; Thais Lima de Souza, Byanca; Makiyama Klosowski, Eduardo; Luiza Ishii-Iwamoto, Emy; Constantin, Jorgete; Polimeni Constantin, Rodrigo.
Afiliação
  • Morais Mewes J; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Rodrigues Silva Gasparin F; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Yoshida T; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Amâncio Daniel da Silva M; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Raquel Marçal Natali M; Department of Morphophysiological Sciences, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Francisco Veiga Bizerra P; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Sayuri Utsunomiya K; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Hideo Gilglioni E; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Shigueaki Mito M; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Cristiane Mantovanelli G; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Thais Lima de Souza B; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Makiyama Klosowski E; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Luiza Ishii-Iwamoto E; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Constantin J; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil.
  • Polimeni Constantin R; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. rpconstantin@uem.br.
Nutrients ; 11(7)2019 Jul 16.
Article em En | MEDLINE | ID: mdl-31315289
Female mice fed a cafeteria diet (FCaf) develop higher liver steatosis and oxidative stress than males (MCaf) as a consequence of unresolved ER stress. Here, we investigated whether mitochondria play a role in this sex difference. The isolated mitochondria from FCaf showed more signs of oxidative stress than those of MCaf, correlated with a reduced content of GSH, increased amount of reactive oxygen species (ROS), and lower activities of enzymes involved in ROS neutralisation. Mitochondria from FCaf and MCaf livers exhibited lower rates of succinate-driven state III respiration and reduced ATPase activity in intact coupled mitochondria compared to their controls fed a standard diet (FC and MC), with no differences between the sexes. Fatty acid oxidation in mitochondria and peroxisomes was higher in MCaf and FCaf compared to their respective controls. In the intact perfused liver, there was no difference between sex or diet regarding the fatty acid oxidation rate. These results indicated that cafeteria diet did not affect mitochondrial energy metabolism, even in FCaf livers, which have higher steatosis and cellular oxidative stress. Nevertheless, the increase in mitochondrial ROS generation associated with a decrease in the antioxidant defence capacity, probably contributes to inducing or reinforcing the ER stress in FCaf livers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Estresse Oxidativo / Dieta / Fígado Gorduroso / Ração Animal / Obesidade Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Estresse Oxidativo / Dieta / Fígado Gorduroso / Ração Animal / Obesidade Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça