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Zein-casein-lysine multicomposite nanoparticles are effective in modulate the intestinal permeability of ferulic acid.
Heep, Graciela; Almeida, Andreia; Marcano, Rossana; Vieira, Daniele; Mainardes, Rubiana Mara; Khalil, Najeh Maissar; Sarmento, Bruno.
Afiliação
  • Heep G; Pharmaceutical Nanotechnology Laboratory, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil; Chemistry Department, Universidade Tecnológica Federal do Paraná, Medianeira, PR, Brazil.
  • Almeida A; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal; INEB - National Institute of Biomedical Engineering, University of Porto, Porto, Portugal; i3S - Institute for Research and Innovation in Health, University of Porto, Porto, Portugal.
  • Marcano R; Pharmaceutical Nanotechnology Laboratory, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
  • Vieira D; Pharmaceutical Nanotechnology Laboratory, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
  • Mainardes RM; Pharmaceutical Nanotechnology Laboratory, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
  • Khalil NM; Pharmaceutical Nanotechnology Laboratory, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
  • Sarmento B; INEB - National Institute of Biomedical Engineering, University of Porto, Porto, Portugal; i3S - Institute for Research and Innovation in Health, University of Porto, Porto, Portugal; CESPU - Institute for Research and Advanced Training in Health Sciences and Technologies, Gandra, Portugal. Electron
Int J Biol Macromol ; 138: 244-251, 2019 Oct 01.
Article em En | MEDLINE | ID: mdl-31279877
The objective of this study was to develop zein-casein-lysine nanoparticles to modulate the intestinal permeability of ferulic acid (FA), a bioactive compound with proven antioxidant properties. The nanoparticles were obtained by a liquid-liquid dispersion method and were characterized in terms of mean size, polydispersity index, zeta potential, association efficiency (AE), in vitro drug release, x-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The in vitro intestinal permeability of nanoparticles was evaluated through Caco-2 and Caco-2/HT29-MTX monoculture and co-culture models, respectively. Nanoparticles presented a mean size of 199 nm and zeta potential of -26 mV. The AE of FA was 23% evaluated by high-performance liquid chromatography (HPLC). XRD showed amorphization of FA after association and FT-IR showed no changes in chemical structures of the compounds after nanoencapsulation. The cytotoxicity assays demonstrated that multicomposite nanoparticles presented a safe profile against Caco-2 and HT29-MTX cells. In the in vitro permeability assay, free FA exhibited higher permeability compared to FA-loaded nanoparticles, possibly due to prolonged FA release from nanoparticles. These new developed zein-casein-lysine nanoparticles may be used for FA sustained delivery by the oral route.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zeína / Caseínas / Ácidos Cumáricos / Nanopartículas / Mucosa Intestinal / Lisina Limite: Humans Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zeína / Caseínas / Ácidos Cumáricos / Nanopartículas / Mucosa Intestinal / Lisina Limite: Humans Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda