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Manganese dioxide coating reduces bacterial adhesion and infection in silicon implants in animal model.
Gon, Lucas Mira; de Campos, Caio César Citatini; Riccetto, Eduardo; Levy, Carlos Emílio; Griguol, Osvaldo; Riccetto, Cássio Luís Zanettini.
Afiliação
  • Gon LM; Division of Urology, Department of Surgery, Faculty of Medical Sciences, University of Campinas, UNICAMP, Rua Vital Brazil, 250, Campinas, SP, 13083-590, Brazil. lucasmgon@gmail.com.
  • de Campos CCC; Division of Urology, Department of Surgery, Faculty of Medical Sciences, University of Campinas, UNICAMP, Rua Vital Brazil, 250, Campinas, SP, 13083-590, Brazil.
  • Riccetto E; Faculty of Medical Sciences, University of Campinas, UNICAMP, Campinas, Brazil.
  • Levy CE; Division of Microbiology of Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, UNICAMP, Campinas, Brazil.
  • Griguol O; Research and Development Department, Ferreyra Industrial Park, Promedon, Córdoba, Argentina.
  • Riccetto CLZ; Division of Urology, Department of Surgery, Faculty of Medical Sciences, University of Campinas, UNICAMP, Rua Vital Brazil, 250, Campinas, SP, 13083-590, Brazil.
World J Urol ; 38(3): 783-788, 2020 Mar.
Article em En | MEDLINE | ID: mdl-31267180
PURPOSE: To propose a new coating to silicone implants using Manganese dioxide. We present bacterial adhesion and proliferation when implants are challenged with Escherichia coli. METHODS: Coated and control silicon implants were placed in two independent subcutaneous pouches in the dorsum of Wistar rats. After skin closure, 0.5 ml of E. coli solution was injected in each incision. The animals were euthanized at 7 and 28 days. Extracted material was cultured and analyzed by confocal microscopy. RESULTS: At 1 week, uncoated implants had a 17-fold higher infection rate (p < 0.001). Coated samples showed a mean bacterial count of 28,700 CFU/ml, while the control ones 503,000 CFU/ml, with a significant mean difference of 474,300 CFU/ml (95% CI 165,900-782,600). At 4 weeks, the mean bacterial growth in coated group was 7600; while in control one was 53,890. The mean difference between groups was 46,200 (95% CI 21,100-71,400). Confocal microscopy presented the percentage of implant's surface with attached bacteria: at 7 days, coated implants had 6.85% and controls 10.9% and the difference was not significant (p =0.32). At 4 weeks, the coated group showed 0.98% of the surface with attached bacteria, while control group showed 7.64%, which resulted in a significant 11-fold difference (p = 0.004). CONCLUSIONS: Manganese dioxide coating inhibits bacterial proliferation and adhesion in subcutaneous silicon implants in an animal model. These findings can be useful to improve development of biomaterials.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Próteses e Implantes / Silicones / Aderência Bacteriana / Infecções Relacionadas à Prótese / Compostos de Manganês / Escherichia coli Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: World J Urol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Próteses e Implantes / Silicones / Aderência Bacteriana / Infecções Relacionadas à Prótese / Compostos de Manganês / Escherichia coli Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: World J Urol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha