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Raman spectroscopy of dorsal root ganglia from streptozotocin-induced diabetic neuropathic rats submitted to photobiomodulation therapy.
Vieira, Willians F; de Magalhães, Silviane F; Farias, Felipe H; de Thomaz, André A; Parada, Carlos A.
Afiliação
  • Vieira WF; Laboratory for Pain Studies, Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
  • de Magalhães SF; Laboratory for Pain Studies, Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
  • Farias FH; Laboratory for Pain Studies, Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
  • de Thomaz AA; Department of Quantum Electronics, Institute of Physics Gleb Wataghin, State University of Campinas (UNICAMP), Campinas, Brazil.
  • Parada CA; Laboratory for Pain Studies, Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
J Biophotonics ; 12(11): e201900135, 2019 11.
Article em En | MEDLINE | ID: mdl-31265175
In this study, we used Raman spectroscopy as a new tool to investigate pathological conditions at the level of chemical bond alterations in biological tissues. Currently, there have been no reports on the spectroscopic alterations caused by diabetic neuropathy in the dorsal root ganglia (DRG). DRG are a target for the treatment of neuropathic pain, and the need for more effective therapies is increasing. Photobiomodulation therapy (PBMT) through infrared low-level laser irradiation (904 nm) has shown analgesic effects on the treatment of neuropathy. Thus, the aim of this study was to use Raman spectroscopy to characterize the spectral DRG identities of streptozotocin (STZ)-induced diabetic neuropathic (hyperalgesic) rats and to study the influence of PBMT over such spectra. Characteristic DRG peaks were identified at 2704, 2850, 2885, 2940, 3061 and 3160 cm-1 , whose assignments are CH2 /CH3 symmetric/asymmetric stretches, and C─H vibrations of lipids and proteins. DRG from hyperalgesic rats showed an increased normalized intensity of 2704, 2850, 2885 and 3160 cm-1 . These same peaks had their normalized intensity reduced after PBMT treatment, accompanied by an anti-hyperalgesic effect. Raman spectroscopy was able to diagnose spectral alterations in DRG of hyperalgesic rats and the PBMT reduced the intensity of hyperalgesia and the altered Raman spectra.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Estreptozocina / Terapia com Luz de Baixa Intensidade / Neuropatias Diabéticas / Gânglios Espinais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Biophotonics Assunto da revista: BIOFISICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Estreptozocina / Terapia com Luz de Baixa Intensidade / Neuropatias Diabéticas / Gânglios Espinais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Biophotonics Assunto da revista: BIOFISICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha