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Effect of bovine non-agglutinating antibodies on the blood clearance of 131I-labelled Brucella abortus strain 45/20.
Parma, A E; Bowden, R A; Santisteban, C G; Cerone, S I; Fernandez, A S.
Afiliação
  • Parma AE; Laboratorio Inmunoquimico, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro, Tandil, Argentina.
Vet Microbiol ; 15(1-2): 121-8, 1987 Oct.
Article em En | MEDLINE | ID: mdl-3125666
Non-agglutinating anti-Brucella abortus S45/20 antibodies were isolated and purified from sera of immunized cattle by means of immunoadsorption and ion-exchange chromatography (DEAE-Sephadex A-50). They corresponded to the IgG1 isotype as shown by immunoelectrophoresis using monospecific anti-IgG1 and anti-bovine gamma globulin sera. These antibodies failed to agglutinate the antigen. They were detected by the anti-bovine gamma globulin test, showing higher titres than those of agglutinating antibodies during the whole period of the experiment. Blood clearance of 131I-S45/20 in mice, was slower in those groups which had received non-agglutinating antibodies than in the control group.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brucella abortus / Brucelose / Anticorpos Antibacterianos Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 1987 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda
Buscar no Google
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PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brucella abortus / Brucelose / Anticorpos Antibacterianos Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 1987 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda