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Calcitriol Inhibits the Proliferation of Triple-Negative Breast Cancer Cells through a Mechanism Involving the Proinflammatory Cytokines IL-1ß and TNF-α.
Martínez-Reza, Isela; Díaz, Lorenza; Barrera, David; Segovia-Mendoza, Mariana; Pedraza-Sánchez, Sigifredo; Soca-Chafre, Giovanny; Larrea, Fernando; García-Becerra, Rocío.
Afiliação
  • Martínez-Reza I; Programa de Investigación de Cáncer de Mama y Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
  • Díaz L; Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14080 Ciudad de México, Mexico.
  • Barrera D; Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14080 Ciudad de México, Mexico.
  • Segovia-Mendoza M; Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14080 Ciudad de México, Mexico.
  • Pedraza-Sánchez S; Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14080 Ciudad de México, Mexico.
  • Soca-Chafre G; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
  • Larrea F; Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14080 Ciudad de México, Mexico.
  • García-Becerra R; Laboratorio de Medicina Personalizada, Instituto Nacional de Cancerología, 14080 Ciudad de México, Mexico.
J Immunol Res ; 2019: 6384278, 2019.
Article em En | MEDLINE | ID: mdl-31093512
Triple-negative breast cancer (TNBC) is one of the most aggressive tumors, with poor prognosis and high metastatic capacity. The aggressive behavior may involve inflammatory processes characterized by deregulation of molecules related to the immunological responses in which interleukin-1ß (IL-1ß) and tumor necrosis factor-α (TNF-α) are involved. It is known that calcitriol, the active vitamin D metabolite, modulates the synthesis of immunological mediators; however, its role in the regulation of IL-1ß and TNF-α in TNBC has been scarcely studied. In the present study, we showed that TNBC cell lines SUM-229PE and HCC1806 expressed vitamin D, IL-1ß, and TNF-α receptors. Moreover, calcitriol, its analogue EB1089, IL-1ß, and TNF-α inhibited cell proliferation. In addition, we showed that synthesis of both IL-1ß and TNF-α was stimulated by calcitriol and its analogue. Interestingly, the antiproliferative activity of calcitriol was significantly abrogated when the cells were treated with anti-IL-1ß receptor 1 (IL-1R1) and anti-TNF-α receptor type 1 (TNFR1) antibodies. Furthermore, the combination of calcitriol with TNF-α resulted in a greater antiproliferative effect than either agent alone, in the two TNBC cell lines and an estrogen receptor-positive cell line. In summary, this study demonstrated that calcitriol exerted its antiproliferative effects in part by inducing the synthesis of IL-1ß and TNF-α through IL-1R1 and TNFR1, respectively, in TNBC cells, highlighting immunomodulatory and antiproliferative functions of calcitriol in TNBC tumors.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcitriol / Fator de Necrose Tumoral alfa / Proliferação de Células / Interleucina-1beta / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: J Immunol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Egito

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcitriol / Fator de Necrose Tumoral alfa / Proliferação de Células / Interleucina-1beta / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: J Immunol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Egito