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Serum albumin saturation test based on non-esterified fatty acids imbalance for clinical employment.
Gonçalves-de-Albuquerque, Cassiano Felippe; Barnese, Marcos Roberto Colombo; Soares, Mariana Alves; Castro-Faria, Mauro Velho; Silva, Adriana Ribeiro; de Castro-Faria-Neto, Hugo Caire; Burth, Patrícia; Younes-Ibrahim, Mauricio.
Afiliação
  • Gonçalves-de-Albuquerque CF; Laboratório de Imunofarmacologia, Departament of Biochemistry, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil; Laboratório de Imunofarmacologia, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
  • Barnese MRC; Laboratório Integrado de Nefrologia, Department of Internal Medicine, Medical Sciences School, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Soares MA; Laboratório de Enzimologia e Sinalização Celular, Department of Cellular and Molecular Biology, Federal Fluminense University, Niteroi, Brazil.
  • Castro-Faria MV; Retired, previously at Laboratório Integrado de Nefrologia, Department of Internal Medicine, Medical Sciences School, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Silva AR; Laboratório de Imunofarmacologia, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
  • de Castro-Faria-Neto HC; Laboratório de Imunofarmacologia, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
  • Burth P; Laboratório de Enzimologia e Sinalização Celular, Department of Cellular and Molecular Biology, Federal Fluminense University, Niteroi, Brazil.
  • Younes-Ibrahim M; Laboratório Integrado de Nefrologia, Department of Internal Medicine, Medical Sciences School, State University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: myounes@terra.com.br.
Clin Chim Acta ; 495: 422-428, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31082361
Fatty acids are fundamental as energy and structural source to the human cells. They are not usually found free in human circulation. Alteration in fatty acids metabolism is linked to diseases such as diabetes, preeclampsia, heart disease, and some infectious diseases. Increased levels of non-esterified fatty acids (NEFA) may cause cell dysfunction and lipotoxicity. Since physiologically fatty acids are transported bound to albumin, we propose here a simple and cheap test that consists of albumin isoelectric focusing determination to measure the potential systemic NEFA cytotoxicity. For validation of this method, albumin isoelectric focusing in 51 serum samples from 40 critically ill patients and 11 controls was compared with NEFA/albumin ratios measured by HPLC. We called this approach an albumin saturation test. This test may indicate to physicians the potential NEFA lipotoxicity guiding them throughout better patient management. The albumin saturation test can point out serum albumin-NEFA saturation through a cheap assay that could be performed by any care facility.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Albumina Sérica / Ácidos Graxos / Focalização Isoelétrica Tipo de estudo: Observational_studies Aspecto: Determinantes_sociais_saude Limite: Humans Idioma: En Revista: Clin Chim Acta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Albumina Sérica / Ácidos Graxos / Focalização Isoelétrica Tipo de estudo: Observational_studies Aspecto: Determinantes_sociais_saude Limite: Humans Idioma: En Revista: Clin Chim Acta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda