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Effects of methylphenidate on the aggressive behavior, serotonin and dopamine levels, and dopamine-related gene transcription in brain of male Nile tilapia (Oreochromis niloticus).
Batalhão, Isabela Gertrudes; Lima, Daína; Russi, Ana Paula Montedor; Boscolo, Camila Nomura Pereira; Silva, Danilo Grunig Humberto; Pereira, Thiago Scremin Boscolo; Bainy, Afonso Celso Dias; de Almeida, Eduardo Alves.
Afiliação
  • Batalhão IG; Department of Chemistry and Environmental Sciences, UNESP - Sao Paulo State University, São Paulo, Brazil.
  • Lima D; Department of Biochemistry, UFSC - Federal University of Santa Catarina, Florianópolis, SP, Brazil.
  • Russi APM; Department of Physiology, UNESP - Sao Paulo State University, Jaboticabal, São Paulo, SP, Brazil.
  • Boscolo CNP; UNIRP - University Center of Rio Preto, São José do Rio Preto, SP, Brazil.
  • Silva DGH; Department of Chemistry and Environmental Sciences, UNESP - Sao Paulo State University, São Paulo, Brazil.
  • Pereira TSB; UNIRP - University Center of Rio Preto, São José do Rio Preto, SP, Brazil.
  • Bainy ACD; FACERES - Morphofunctional Laboratory, FACERES Medical School, São José do Rio Preto, SP, Brazil.
  • de Almeida EA; Department of Biochemistry, UFSC - Federal University of Santa Catarina, Florianópolis, SP, Brazil.
Fish Physiol Biochem ; 45(4): 1377-1391, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31054043
The occurrence of pharmaceuticals in the aquatic environment has increased considerably in the last decades, causing negative biochemical, physiological, and behavioral effects in aquatic organisms. In this study, we evaluated the effects of methylphenidate (MPH) on the aggressive behavior, dopamine-related gene transcript levels, monoamine levels, and carboxylesterase transcript levels and activity in the brain of male Nile tilapia (Oreochromis niloticus). Carboxylesterase activity was also measured in the liver and gills. Fish were exposed for 5 days to MPH at 20 and 100 ng L-1. Fish exposed to 100 ng L-1 of MPH showed increased aggressiveness and decreased dopamine (DA) and serotonin (5-HT) levels. No changes were observed in plasma testosterone levels and in the transcript levels of D1 and D2 dopamine receptors, dopamine transporter (DAT), and carboxylesterase 2 (CES2). Exposure to 100 ng L-1 of MPH caused a decrease in the transcript levels of carboxylesterase 3 (CES3) and an increase in tyrosine hydroxylase (TH), while exposure to 20 ng L-1 of MPH increased the transcript levels of D5 dopamine receptor. Carboxylesterase activity was unchanged in the brain and liver and increased in the gills of fish exposed to 20 ng L-1. These results indicate that MPH at 100 ng L-1 increases aggressiveness in Nile tilapia, possibly due to a decrease in 5-HT levels in the brain and alterations in dopamine levels and dopamine-related genes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Inibidores da Captação de Dopamina / Ciclídeos / Metilfenidato Limite: Animals Idioma: En Revista: Fish Physiol Biochem Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Inibidores da Captação de Dopamina / Ciclídeos / Metilfenidato Limite: Animals Idioma: En Revista: Fish Physiol Biochem Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda