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Effect of Two Different Isolates of Leishmania mexicana in the Production of Cytokines and Phagocytosis by Murine Dendritic Cells.
Rivera-Fernández, Ilse; Argueta-Donohué, Jesús; Wilkins-Rodríguez, Arturo A; Gutiérrez-Kobeh, Laila.
Afiliação
  • Rivera-Fernández I; 1 Unidad de Investigación UNAM-INC, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México. Juan Badiano no. 1. Col. Belisario Domínguez, sección XVI, cp 14080, Ciudad de México, México.
  • Argueta-Donohué J; 2 Instituto Nacional de Psiquiatría, Calzada México-Xochimilco 101, Huipulco, cp 14370 Ciudad de México, México.
  • Wilkins-Rodríguez AA; 1 Unidad de Investigación UNAM-INC, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México. Juan Badiano no. 1. Col. Belisario Domínguez, sección XVI, cp 14080, Ciudad de México, México.
  • Gutiérrez-Kobeh L; 1 Unidad de Investigación UNAM-INC, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México. Juan Badiano no. 1. Col. Belisario Domínguez, sección XVI, cp 14080, Ciudad de México, México.
J Parasitol ; 105(2): 359-370, 2019 04.
Article em En | MEDLINE | ID: mdl-31033389
Species of the genus Leishmania are the causal agents of leishmaniasis, a disease with diametrically different clinical manifestations that have been attributed to the species and host immune response. Some Leishmania species, including Leishmania mexicana, are capable of causing both localized cutaneous leishmaniasis (LCL) and diffuse cutaneous leishmaniasis (DCL). Therefore, it is possible that intraspecific differences may exist that contribute to the development of distinct clinical forms. Dendritic cells (DC) are important host cells of Leishmania spp. parasites, and cytokine production and phagocytosis upon infection with the parasite are significant for the outcome of the disease. In the present study we analyzed the production of IL-12, TNF-α, and IL-10 by DC infected with L. mexicana amastigotes isolated from a patient with LCL (amastigote = Lac) and from a patient with DCL (amastigote = Diact) by murine DC. Furthermore, we compared the frequency of phagocytosis of L. mexicana amastigotes of each isolate by fluorescence and optical microscopy and by flow cytometry. We show that the infection of DC with Diact amastigotes elicited the secretion of IL-10, TNF-α, and IL-12 by DC to a major extent as compared to the infection with Lac amastigotes. On the other hand, Lac and Diact amastigotes were similarly phagocytosed by DC, but interestingly there were more vacuoles in DC infected with Diact amastigotes. Our results suggest that isolates from a same species of Leishmania, such as L. mexicana, with different degrees of virulence according to the clinical manifestation they cause, differ in their capacity to elicit cytokine production and form vacuoles in DC.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Células Dendríticas / Leishmania mexicana / Células da Medula Óssea / Citocinas Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: J Parasitol Ano de publicação: 2019 Tipo de documento: Article País de publicação: Estados Unidos
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Células Dendríticas / Leishmania mexicana / Células da Medula Óssea / Citocinas Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: J Parasitol Ano de publicação: 2019 Tipo de documento: Article País de publicação: Estados Unidos