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Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae.
Fernandes, Kelly; Gomes, Andreia; Calado, Leonardo; Yasui, George; Assis, Diego; Henry, Theodore; Fonseca, Ana; Pinto, Ernani.
Afiliação
  • Fernandes K; Natural Resources Institute, Federal University of Itajubá, 1303 BPS Avenue, Itajubá, MG 37500-903, Brazil. kelly.af@usp.br.
  • Gomes A; School of Pharmaceutical Sciences, University of Sao Paulo, 580 Professor Lineu Prestes Avenue, São Paulo, SP 05508-000, Brazil. kelly.af@usp.br.
  • Calado L; Natural Resources Institute, Federal University of Itajubá, 1303 BPS Avenue, Itajubá, MG 37500-903, Brazil. andreia.gomes@ifrj.edu.br.
  • Yasui G; Federal Institute of Education Science and Technology of Rio de Janeiro, Washington Luis Highway, Niteroi, RJ 24310-000, Brazil. andreia.gomes@ifrj.edu.br.
  • Assis D; National Center for Research and Conservation of Continentals' Fish-CEPTA, SP-201 (Pref. Euberto Nemésio Pereira de Godoy-Motorway), Km 6.5, Pirassununga, SP 13630-970, Brazil. leonardocalado@hotmail.com.
  • Henry T; Faculty of Technology, State University of Campinas, 1888 Paschoal Marmo Street, Limeira, SP 13484-332, Brazil. leonardocalado@hotmail.com.
  • Fonseca A; National Center for Research and Conservation of Continentals' Fish-CEPTA, SP-201 (Pref. Euberto Nemésio Pereira de Godoy-Motorway), Km 6.5, Pirassununga, SP 13630-970, Brazil. yasui@usp.br.
  • Pinto E; Bruker Daltonics Corporation, Condomínio BBP-Barão de Mauá, Atibaia, SP 12954-260, Brazil. diego.assis@bruker.com.
Toxins (Basel) ; 11(4)2019 04 13.
Article em En | MEDLINE | ID: mdl-31013880
Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence and the rupturing of cells during bloom episodes. We determined the toxicity of cyanopeptides identified from two strains of Microcystis (M. panniformis MIRS-04 and M. aeruginosa NPDC-01) in a freshwater tropical fish, Astyanax altiparanae (yellowtail tetra, lambari). Aqueous extracts of both Microcystis strains were prepared in order to simulate realistic fish exposure to these substances in a freshwater environment. Both strains were selected because previous assays evidenced the presence of microcystins (MCs) in MIRS-04 and lack of cyanotoxins in NPDC-01. Identification of cyanobacterial secondary metabolites was performed by LC-HR-QTOF-MS and quantification of the MC-LR was carried out by LC-QqQ-MS/MS. MIRS-04 produces the MCs MC-LR, MC-LY and MC-HilR as well as micropeptins B, 973, 959 and k139. NPCD-01 biosynthetizes microginins FR1, FR2/FR4 and SD-755, but does not produce MCs. Larval fish survival and changes in morphology were assessed for 96 h exposure to aqueous extracts of both strains at environmentally relevant concentrations from 0.1 to 0.5 mg (dry weight)/mL, corresponding to 0.15 to 0.74 µg/mL of MC-LR (considering dried amounts of MIRS-04 for comparison). Fish mortality increased with concentration and time of exposure for both strains of Microcystis. The frequencies of morphological abnormalities increased with concentration in both strains, and included abdominal and pericardial oedema, and spinal curvature. Results demonstrate that toxicity was not solely caused by MCs, other classes of cyanobacterial secondary metabolites contributed to the observed toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Toxinas Bacterianas / Poluentes Químicos da Água / Microcystis / Characidae / Larva Limite: Animals Idioma: En Revista: Toxins (Basel) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Toxinas Bacterianas / Poluentes Químicos da Água / Microcystis / Characidae / Larva Limite: Animals Idioma: En Revista: Toxins (Basel) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça