ABSTRACT: Figueiredo, DH, Figueiredo, DH, Moreira, A, Gonçalves, HR, and Dourado, AC. Dose-response relationship between internal training load and changes in performance during the preseason in youth soccer players. J Strength Cond Res 35(8): 2294-2301, 2021-The aim of this study was to describe training intensity distribution based on the session rating of perceived exertion (sRPE) and heart rate (HR) methods and examine the dose-response relation between internal training load (ITL) and change in performance of 16 youth soccer players (mean ± SD age: 18.75 ± 0.68 years, height: 175.3 ± 5.5 cm, body mass: 68.7 ± 6.5 kg, and body fat: 10.7 ± 1.2%) belonging to a Brazilian first division team during a 3-week preseason. The sRPE and HR data were registered daily to calculate the ITL and the training intensity distribution, in 3 intensity zones (low, moderate, and high). The Yo-yo Intermittent Recovery Level 1 (Yo-yo IR1) was evaluated before and after experimental period. The total time spent in the low-intensity zone (HR method) was greater (p < 0.01) compared with the moderate- and high-intensity zones. No difference was observed between training intensity zones determined by the sRPE method (p > 0.05). Negative correlations were observed between weekly mean sRPE-TL (r = -0.69), Edward's-TL (r = -0.50), and change in Yo-yo IR1. Linear regression indicated that weekly mean sRPE-TL (F1;14 = 13.3; p < 0.01) and Edward's-TL (F1;14 = 4.8; p < 0.05) predicted 48.7 and 25.5% of the variance in performance change, respectively. Stepwise linear regression revealed that these 2-predictor variables (F2;13 = 18.9; p < 0.001) explained 74.5% of the variance in performance change. The results suggest that the sRPE and HR methods cannot be used interchangeably to determine training intensity distribution. Moreover, sRPE-TL seems to be more effective than the HR-based TL method to predict changes in performance in youth soccer players.