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New bioanalytical platform based on the use of avidin for the successful exfoliation of multi-walled carbon nanotubes and the robust anchoring of biomolecules. Application for hydrogen peroxide biosensing.
Gutierrez, F A; Rubianes, M D; Rivas, G A.
Afiliação
  • Gutierrez FA; INFIQC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre S/N, Ciudad Universitaria, 5000, Córdoba, Argentina.
  • Rubianes MD; INFIQC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre S/N, Ciudad Universitaria, 5000, Córdoba, Argentina. Electronic address: rubianes@fcq.unc.edu.ar.
  • Rivas GA; INFIQC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre S/N, Ciudad Universitaria, 5000, Córdoba, Argentina. Electronic address: grivas@fcq.unc.edu.ar.
Anal Chim Acta ; 1065: 12-20, 2019 Aug 13.
Article em En | MEDLINE | ID: mdl-31005144
We are reporting an innovative building-block for the development of biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with avidin (MWCNTs-avidin). In this work, at variance with previous reports, avidin has the double role of simultaneously being the exfoliating agent of MWCNTs and the platform for anchoring different biotinylated biomolecules. The optimum dispersion was obtained by sonicating for 5.0 min 0.50 mgmL-1 MWCNTs with 1.00 mgmL-1 avidin solution prepared in 50:50 v/v ethanol/water. As proof-of-concept, we immobilized biotinylated horseradish peroxidase (b-HRP) at glassy carbon electrodes (GCE) modified with MWCNTs-avidin to develop a hydrogen peroxide biosensor using hydroquinone as redox mediator. Surface plasmon resonance, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry demonstrated that, even after the partial denaturation of avidin due to the drastic conditions used to functionalize the MWCNTs, it preserves the biorecognition properties and efficiently interacts with biotinylated horseradish peroxidase (b-HRP). The analytical characteristics of the resulting hydrogen peroxide biosensor are the following: linear range between 1.0 × 10-6 M and 1.4 × 10-5 M, sensitivity of (1.37 ±â€¯0.04) x 105 µAM-1, detection limit of 24 nM and reproducibility of 2.9%. The sensor was challenged with different samples, a mouthwash solution, human blood serum and milk, with very good performance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Avidina / Nanotubos de Carbono / Técnicas Eletroquímicas / Peroxidase do Rábano Silvestre / Peróxido de Hidrogênio Limite: Animals / Humans Idioma: En Revista: Anal Chim Acta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Avidina / Nanotubos de Carbono / Técnicas Eletroquímicas / Peroxidase do Rábano Silvestre / Peróxido de Hidrogênio Limite: Animals / Humans Idioma: En Revista: Anal Chim Acta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda