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Dynamic expression of glial fibrillary acidic protein and ionized calcium binding adaptor molecule 1 in the mouse spinal cord dorsal horn under pathological pain states.
Turcato, Flavia; Almeida, Cayo; Mota, Clarissa; Kusuda, Ricardo; Carvalho, Andrea; Nascimento, Glauce C; Zanon, Sonia; Leite-Panissi, Christie R; Lucas, Guilherme.
Afiliação
  • Turcato F; a Department of Physiology, Laboratory of Pain Neurobiology, Ribeirão Preto School of Medicine , University of São Paulo , Ribeirão Preto , Brazil.
  • Almeida C; b Federal University of ABC , São Bernardo do Campo , Brazil.
  • Mota C; a Department of Physiology, Laboratory of Pain Neurobiology, Ribeirão Preto School of Medicine , University of São Paulo , Ribeirão Preto , Brazil.
  • Kusuda R; a Department of Physiology, Laboratory of Pain Neurobiology, Ribeirão Preto School of Medicine , University of São Paulo , Ribeirão Preto , Brazil.
  • Carvalho A; c Department of Experimental Psychology, Neuroscience and Behavior Training Program , Institute of Psychology, University of São Paulo , São Paulo , Brazil.
  • Nascimento GC; d Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry School , University of São Paulo , Ribeirão Preto , Brazil.
  • Zanon S; a Department of Physiology, Laboratory of Pain Neurobiology, Ribeirão Preto School of Medicine , University of São Paulo , Ribeirão Preto , Brazil.
  • Leite-Panissi CR; d Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry School , University of São Paulo , Ribeirão Preto , Brazil.
  • Lucas G; a Department of Physiology, Laboratory of Pain Neurobiology, Ribeirão Preto School of Medicine , University of São Paulo , Ribeirão Preto , Brazil.
Neurol Res ; 41(7): 633-643, 2019 Jul.
Article em En | MEDLINE | ID: mdl-31002029
Objective: Animal models of chronic pain have demonstrated that glial cells are promising target for development of analgesic drugs. However, preclinical studies on glial response under chronic pain conditions vary depending on the cellular markers, the species used, the experimental design and model. Therefore, we investigate the expression profile of GFAP and Iba-1 during the behavioral manifestation of sensory disorder in inflammatory and neuropathic pain models. Methods: the expression profile of fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule-1 (Iba-1) were quantitated in the spinal dorsal horn of Balb/C mice submitted to six models of chronic pain. Protein analysis was performed by western blot and the results colligated with pain-related behavior. Results: Using the same method to quantitate proteins we observed that while GFAP is upregulated after axotomy, partial nerve injury and cutaneous inflammation, its expression is not changed during muscle inflammation, non-inflammatory muscle pain, and in a viral-associated pain. Differently, Iba-1 is downregulated after axotomy but upregulated after partial lesion of peripheral nerve as well as after virus inoculation and during non-inflammatory muscle pain. Cutaneous and muscle inflammation induced no change in Iba-1 expression in the dorsal horn.In spite of a marked time-dependent variation in protein expression, mechanical allodynia was present at any time of all the models investigated. Discussion: Under distinct pain conditions, GFAP and Iba-1 expression is dependent on the origin of the stimulus, disease progression and tissue affected. Moreover, their expression and is not necessarily associated to the behavior manifestation of pain.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação ao Cálcio / Dor Crônica / Corno Dorsal da Medula Espinal / Proteína Glial Fibrilar Ácida / Inflamação / Proteínas dos Microfilamentos / Neuralgia Limite: Animals Idioma: En Revista: Neurol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação ao Cálcio / Dor Crônica / Corno Dorsal da Medula Espinal / Proteína Glial Fibrilar Ácida / Inflamação / Proteínas dos Microfilamentos / Neuralgia Limite: Animals Idioma: En Revista: Neurol Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido