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SME-4-producing Serratia marcescens from Argentina belonging to clade 2 of the S. marcescens phylogeny.
Dabos, Laura; Patiño-Navarrete, Rafael; Nastro, Marcela; Famiglietti, Angela; Glaser, Philippe; Rodriguez, Carlos H; Naas, Thierry.
Afiliação
  • Dabos L; EA7361 'Structure, dynamic, function and expression of broad spectrum ß-lactamases', Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France.
  • Patiño-Navarrete R; Joint research Unit EERA 'Evolution and Ecology of Resistance to Antibiotics', Institut Pasteur-APHP-University Paris Sud, Paris, France.
  • Nastro M; Joint research Unit EERA 'Evolution and Ecology of Resistance to Antibiotics', Institut Pasteur-APHP-University Paris Sud, Paris, France.
  • Famiglietti A; Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
  • Glaser P; Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
  • Rodriguez CH; Joint research Unit EERA 'Evolution and Ecology of Resistance to Antibiotics', Institut Pasteur-APHP-University Paris Sud, Paris, France.
  • Naas T; CNRS, UMR3525, Paris, France.
J Antimicrob Chemother ; 74(7): 1836-1841, 2019 07 01.
Article em En | MEDLINE | ID: mdl-30993333
BACKGROUND: SME carbapenemases are increasingly reported, especially from North and South America. Here, we describe an SME-4-producing Serratia marcescens (SME-Sm) clinical isolate from Argentina and compare its genome with other SME-Sm and Sm isolates recovered from public databases. METHODS: Sm isolates were characterized by WGS using Illumina technology, susceptibility testing and MIC determination. Carbapenemase activity was revealed by biochemical tests based on imipenem hydrolysis. A whole-genome phylogeny was estimated for all the Sm isolates retrieved from public databases with kSNP3 and a whole-genome phylogenetic analysis based on non-recombinant core SNPs was inferred for Sm complete genomes and for those encoding any blaSME variants. RESULTS: Sm163 was resistant to amoxicillin, temocillin, aztreonam and carbapenems, remaining susceptible to extended-spectrum cephalosporins. WGS analysis of Sm163 revealed a genome of 5139329 bp and a chromosomally encoded blaSME-4 carbapenemase gene located on a genomic island closely related to SmarGI1-1 of Sm N11-02820. Comparison of the Sm genomes revealed that the 14 SME-Sm isolates possess this genomic island inserted at the same loci, that 13/14 belong to clade 1 and that 11/14 form a well-defined subcluster of cluster I of Sm clade 1, while Sm163 belongs to clade 2, suggesting that an SME-encoding genomic island may have been transferred between isolates from different clades. CONCLUSIONS: To the best of our knowledge this is the first report of an SME-4-encoding Sm from Argentina. The blaSME-4 gene is located on a SmarGI1-1-like genomic island. The genome of Sm163 belongs to clade 2, unlike all the other SME-Sm isolates, which belong to clade 1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Proteínas de Bactérias / Beta-Lactamases / Infecções por Serratia / Enterobacteriáceas Resistentes a Carbapenêmicos / Genótipo Limite: Humans / Male / Middle aged País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Antimicrob Chemother Ano de publicação: 2019 Tipo de documento: Article País de afiliação: França País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Proteínas de Bactérias / Beta-Lactamases / Infecções por Serratia / Enterobacteriáceas Resistentes a Carbapenêmicos / Genótipo Limite: Humans / Male / Middle aged País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Antimicrob Chemother Ano de publicação: 2019 Tipo de documento: Article País de afiliação: França País de publicação: Reino Unido