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Temporal analysis of distribution pattern of islet cells and antioxidant enzymes for diabetes onset in postnatal critical development window in rats.
Gallego, Franciane Quintanilha; Miranda, Carolina Abreu; Sinzato, Yuri Karen; Iessi, Isabela Lovizutto; Dallaqua, Bruna; Pando, Rogelio Hernandez; Rocha, Noeme Sousa; Volpato, Gustavo Tadeu; Damasceno, Débora Cristina.
Afiliação
  • Gallego FQ; Laboratory of Experimental Research of Gynecology and Obstetrics, Postgraduate Course of Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Miranda CA; Laboratory of Experimental Research of Gynecology and Obstetrics, Postgraduate Course of Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Sinzato YK; Laboratory of Experimental Research of Gynecology and Obstetrics, Postgraduate Course of Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Iessi IL; Laboratory of Experimental Research of Gynecology and Obstetrics, Postgraduate Course of Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Dallaqua B; DeVry Ruy Barbosa School (DeVry Brazil Group), Salvador, Bahia State, Brazil.
  • Pando RH; Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubirán", Mexico City, Mexico.
  • Rocha NS; Department of Pathology, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Volpato GT; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil.
  • Damasceno DC; Laboratory of Experimental Research of Gynecology and Obstetrics, Postgraduate Course of Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil. Electronic address: damascenofmb@gmail.com.
Life Sci ; 226: 57-67, 2019 Jun 01.
Article em En | MEDLINE | ID: mdl-30930115
AIM: At performing a temporal analysis of the distribution pattern of islet endocrine cells and antioxidant enzymes in diabetic rats during the post-natal critical development window. MAIN METHODS: The newborns received streptozotocin (STZ) at birth for diabetes induction, and control females received the vehicle. The animals were euthanized at different lifetimes: D5, D10, D15, and D30. Morphological analysis of pancreas and biochemical assays was performed. KEY FINDINGS: The STZ-induced rats presented irregular shape of islet on D5 and there was an attempt to restore of this shape in other life moment studied. There was an increase progressive in islet area, however they maintained smaller than those of control rats, with lower labeling intensity for insulin, higher for glucagon and somatostatin, lower for SOD-1 was lower in the islets of the STZ-induced animals at all times studied and for GSH-Px in D10 and D30. SIGNIFICANCE: Although STZ-induced diabetic rats presented compensatory mechanisms to restore the mass of endocrine cells, this was not sufficient since these rats developed the diabetic state. This was confirmed by the oral glucose tolerance test from D30. In addition, the delta (δ)-cells presented ectopic location in islets, indicating a possible relationship for beta (ß)-cell mass restoration. There was a response of the pancreas to reduce the hyperglycemia in the first month of life. Furthermore, the cells from the endocrine pancreas of diabetic animals show a decline of antioxidant enzymatic, contributing to the increased susceptibility of cells to hyperglycemia-induced ROS in this postnatal critical development window.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ilhotas Pancreáticas / Diabetes Mellitus Experimental Limite: Animals / Pregnancy Idioma: En Revista: Life Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ilhotas Pancreáticas / Diabetes Mellitus Experimental Limite: Animals / Pregnancy Idioma: En Revista: Life Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda