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Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM).
Gutiérrez, Sergio; Correa, Adriana; Hernández-Gómez, Cristhian; De La Cadena, Elsa; Pallares, Christian; Villegas, María Virginia.
Afiliação
  • Gutiérrez S; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia.
  • Correa A; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Universidad Santiago de Cali, Cali, Colombia.
  • Hernández-Gómez C; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Grupo de Investigación en Resistencia Antimicrobiana y Epidemiología Hospitalaria - RAEH, Universidad El Bosque, Bogotá, Colombia.
  • De La Cadena E; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Grupo de Investigación en Resistencia Antimicrobiana y Epidemiología Hospitalaria - RAEH, Universidad El Bosque, Bogotá, Colombia.
  • Pallares C; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Grupo de Investigación en Resistencia Antimicrobiana y Epidemiología Hospitalaria - RAEH, Universidad El Bosque, Bogotá, Colombia.
  • Villegas MV; Bacterial Resistance and Hospital Epidemiology Unit, International Center for Medical Research and Training (CIDEIM), Cali, Colombia; Grupo de Investigación en Resistencia Antimicrobiana y Epidemiología Hospitalaria - RAEH, Universidad El Bosque, Bogotá, Colombia. Electronic address: mariavirginia.v
Enferm Infecc Microbiol Clin (Engl Ed) ; 37(10): 648-651, 2019 Dec.
Article em En, Es | MEDLINE | ID: mdl-30898368
INTRODUCTION: The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem. METHODS: We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10µL loop) in 400µL water, in which a 10µg disk of meropenem/imipenem is immersed. After 2h of incubation (35°C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19mm, positive; ≥25mm negative; 20-24mm, undetermined. RESULTS: Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively). CONCLUSIONS: The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Proteínas de Bactérias / Beta-Lactamases / Carbapenêmicos / Meropeném / Antibacterianos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En / Es Revista: Enferm Infecc Microbiol Clin (Engl Ed) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Espanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Proteínas de Bactérias / Beta-Lactamases / Carbapenêmicos / Meropeném / Antibacterianos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En / Es Revista: Enferm Infecc Microbiol Clin (Engl Ed) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Espanha