Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay.
Rev Soc Bras Med Trop
; 52: e20180300, 2019 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-30892548
INTRODUCTION: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS: CESDA could be further explored for sepsis diagnosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores
/
Quimiotaxia
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Caenorhabditis elegans
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Sepse
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Rev Soc Bras Med Trop
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Malásia
País de publicação:
Brasil