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Prevalence of human papillomavirus (HPV) in the semen of patients submitted to assisted reproductive technology treatment in a private clinic in Brazil.
Bossi, Renata de Lima; Valadares, Jéssica Bruna Fernandes; Puerto, Helen Lima Del; Rocha, Maria Gabrielle Lima; Braga, Letícia Conceição; Sampaio, Marcos Aurelio Coelho; França, Patrícia Pinho; Alvarenga, Débora Moreira; Geber, Selmo.
Afiliação
  • Bossi RL; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
  • Valadares JBF; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
  • Puerto HLD; Departamento de Patologia Geral do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais- Brazil.
  • Rocha MGL; Departamento de Análises Clinicas e Toxicológicas da Faculdade de Farmácia da Universidade Federal de Minas Gerais - Brazil.
  • Braga LC; FUNED - Fundação Ezequiel Dias- Belo Horizonte/MG- Brazil.
  • Sampaio MAC; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
  • França PP; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
  • Alvarenga DM; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
  • Geber S; Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
JBRA Assist Reprod ; 23(3): 205-209, 2019 08 22.
Article em En | MEDLINE | ID: mdl-30875170
OBJECTIVE: The aim of our study was to identify the prevalence of HPV in the semen of men submitted to ART treatment and look into the possible impacts of the virus on sperm parameters. METHODS: Thirty-five patients treated for infertility from March to August 2016 were invited to join the study. Samples with a minimum concentration of 40x106 spermatozoa per milliliter were included in the study. After the evaluation of semen parameters, DNA extraction and PCR were performed to verify the presence of HPV by electrophoresis in 8% polyacrylamide gel. RESULTS: Patient age ranged from 27 to 68 years (mean 39.2 years). Semen analysis showed a mean volume of 2.5mL; mean concentration of 58.9x106; and mean motility of 51.8%. HPV DNA was identified in seven semen samples from 25 patients (28%). Ten samples with DNA concentrations below 10ng/µL were excluded from the study due to poor amplification quality. There was no statistical difference in sperm concentration when HPV-negative and HPV-positive samples were compared (65.9x106 vs. 62.3x106; p=0.70). However, sperm motility was significantly higher in HPV-positive semen (65% vs. 46.6%; p=0.02). CONCLUSIONS: HPV prevalence was 28% in the semen of patients submitted to ART treatment. HPV-positive samples had statistically increased motility compared to negative samples (65% vs. 46.6%; p=0.02).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papillomaviridae / Sêmen / Infecções por Papillomavirus / Técnicas de Reprodução Assistida / Infertilidade Masculina Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adult / Aged / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papillomaviridae / Sêmen / Infecções por Papillomavirus / Técnicas de Reprodução Assistida / Infertilidade Masculina Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adult / Aged / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil