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The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm-zona pellucida binding.
Anchordoquy, Juan Patricio; Anchordoquy, Juan Mateo; Lizarraga, Raúl Martín; Nikoloff, Noelia; Pascua, Ana Malen; Furnus, Cecilia Cristina.
Afiliação
  • Anchordoquy JP; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
  • Anchordoquy JM; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
  • Lizarraga RM; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
  • Nikoloff N; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
  • Pascua AM; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
  • Furnus CC; 1Igevet e Instituto de Genética Veterinaria 'Prof. Fernando N. Dulout' (UNLP-Conicet La Plata),Facultad de Ciencias Veterinarias,Universidad Nacional de La Plata,calle 60 y 118 s/n,CP 1900,La Plata,Buenos Aires,Argentina.
Zygote ; 27(2): 89-96, 2019 Apr.
Article em En | MEDLINE | ID: mdl-30871652
SummarySperm-zona pellucida (ZP) binding is a necessary event for successful fertilization. The aim of this study was to determine the effect of trace minerals such as copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) on bovine spermatozoa binding to ZP. Sperm viability, functional membrane integrity, acrosomal status (AS), total antioxidant capacity (TAC) and sperm lipid peroxidation (LPO) were also evaluated. For the present study, in vitro fertilization (IVF) medium was supplemented with Cu (0.4 µg/ml Cu), Mn (5 ng/ml Mn), Se (100 ng/ml Se), Zn (0.8 µg/ml Zn), all minerals (Cu+Mn+Se+Zn), or tested without supplement (Control). Considerably more sperm bound to ZP when Cu, Se or Zn were added to the IVF medium, but there were no difference compared with the Control, Mn and Cu+Mn+Se+Zn groups. After 1 h of incubation, viability was increased by the addition of Cu, Mn and Se with respect to the Control but, after 2 h, viability was higher only with the addition of Mn to IVF medium. Functional membrane integrity improved in sperm treated with Cu. Acrosome integrity was higher in sperm treated with Zn after 1 h of incubation. LPO was significantly higher in sperm treated with Cu or Cu+Mn+Se+Zn. The mean TACs of sperm treated with Cu, Mn, Zn or Cu+Mn+Se+Zn were lower than in the Control. In conclusion, the results obtained in the present study determined that the presence of Cu, Se and Zn in the IVF medium increased the number of spermatozoa bound to the ZP, highlighting the importance of these minerals in the fertilization process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interações Espermatozoide-Óvulo / Espermatozoides / Oligoelementos / Zona Pelúcida Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interações Espermatozoide-Óvulo / Espermatozoides / Oligoelementos / Zona Pelúcida Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido