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Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication.
Reyes-Ruiz, José Manuel; Osuna-Ramos, Juan Fidel; Bautista-Carbajal, Patricia; Jaworski, Elizabeth; Soto-Acosta, Rubén; Cervantes-Salazar, Margot; Angel-Ambrocio, Antonio H; Castillo-Munguía, Juan Pablo; Chávez-Munguía, Bibiana; De Nova-Ocampo, Mónica; Routh, Andrew; Del Ángel, Rosa María; Salas-Benito, Juan Santiago.
Afiliação
  • Reyes-Ruiz JM; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
  • Osuna-Ramos JF; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
  • Bautista-Carbajal P; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
  • Jaworski E; Department of Biochemistry and Molecular Biology, The University of Texas, Medical Branch, Galveston, TX 77555, USA.
  • Soto-Acosta R; Department of Biochemistry and Molecular Biology, The University of Texas, Medical Branch, Galveston, TX 77555, USA.
  • Cervantes-Salazar M; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
  • Angel-Ambrocio AH; División de Investigación, Facultad de Medicina, Universidad Autónoma de México, Mexico.
  • Castillo-Munguía JP; Maestría en Ciencias en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico.
  • Chávez-Munguía B; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico.
  • De Nova-Ocampo M; Maestría en Ciencias en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico.
  • Routh A; Department of Biochemistry and Molecular Biology, The University of Texas, Medical Branch, Galveston, TX 77555, USA; Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Del Ángel RM; Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico. Electronic address: rmangel@cinvestav.mx.
  • Salas-Benito JS; Maestría en Ciencias en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico; Doctorado en Ciencias en Biotecnología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico. Electronic address: jsalas@ipn.mx.
Virology ; 531: 1-18, 2019 05.
Article em En | MEDLINE | ID: mdl-30844508
Dengue viruses (DENV) are important arboviruses that can establish a persistent infection in its mosquito vector Aedes. Mosquitoes have a short lifetime in nature which makes trying to study the processes that take place during persistent viral infections in vivo. Therefore, C6/36 cells have been used to study this type of infection. C6/36 cells persistently infected with DENV 2 produce virions that cannot infect BHK -21 cells. We hypothesized that the following passages in mosquito cells have a deleterious impact on DENV fitness in vertebrate cells. Here, we demonstrated that the viral particles released from persistently infected cells were infectious to mosquito but not to vertebrate cells. This host restriction occurs at the replication level and is associated with several mutations in the DENV genome. In summary, our findings provide new information about viral replication fitness in a host-dependent manner.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Replicação Viral / Aedes / Vírus da Dengue / Especificidade de Hospedeiro / Mosquitos Vetores Limite: Animals Idioma: En Revista: Virology Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Replicação Viral / Aedes / Vírus da Dengue / Especificidade de Hospedeiro / Mosquitos Vetores Limite: Animals Idioma: En Revista: Virology Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos