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Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats1.
Morsoleto, Maria Jose Misael da Silva; Sella, Valeria; Machado, Paula; Bomfim, Fernando do; Fernandes, Maria Helena; Morgado, Fernando; Lopes Filho, Gaspar de Jesus; Plapler, Helio.
Afiliação
  • Morsoleto MJMDS; Postdoctoral, Postgraduate Program in Interdisciplinary Surgical Sciences, Universidade Federal de São Paulo (UNIFESP), Brazil. Design, intellectual and scientific content of the study; acquisition and interpretation of data; manuscript preparation and writing.
  • Sella V; Fellow PhD degree, Postgraduate Program in Interdisciplinary Surgical Science, UNIFESP, Sao Paulo-SP, Brazil. Conception and design of the study.
  • Machado P; Physiotherapist, Postgraduate Program in Interdisciplinary Surgical Sciences, UNIFESP, Sao Paulo-SP, Brazil. Technical procedures.
  • Bomfim FD; Fellow PhD degree, Postgraduate Program in Interdisciplinary Surgical Sciences, UNIFESP, Sao Paulo-SP, Brazil. Technical procedures.
  • Fernandes MH; Associate Professor, Department of Pharmacology and Cellular Compatibility, Dental Medicine Faculty, Universidade do Porto, Portugal. Histopathological examinations, Analysis and interpretation of data.
  • Morgado F; Associate Professor, Department of Biology, Universidade de Aveiro, Portugal. Analysis and interpretation of data, statistics analysis.
  • Lopes Filho GJ; Associate Professor, Department of Surgery, Medical School, UNIFESP, Sao Paulo-SP, Brazil. Critical revision, final approval.
  • Plapler H; Associate Professor, Department of Surgery, Medical School, UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific content of the study; critical revision.
Acta Cir Bras ; 34(2): e201900210, 2019 Feb 28.
Article em En | MEDLINE | ID: mdl-30843943
PURPOSE: To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser. METHODS: Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of: alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction. RESULTS: At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group. CONCLUSION: The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Diferenciação Celular / Sobrevivência Celular / Terapia com Luz de Baixa Intensidade Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Diferenciação Celular / Sobrevivência Celular / Terapia com Luz de Baixa Intensidade Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil