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Bone demineralization promotes superior spread of preosteoblast in culture.
Manfredi, Gustavo Gonçalves do Prado; Tokuhara, Cíntia Kazuko; Salmeron, Samira; de Carvalho, Érika Beatriz Spada; Liboa-Filho, Paulo Noronha; Damante, Carla Andreotti; Sant'Ana, Adriana Campos Passanezi; Zangrando, Mariana Schutzer Ragghianti; Greghi, Sebastião Luis Aguiar; de Rezende, Maria Lúcia Rubo.
Afiliação
  • Manfredi GGDP; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Tokuhara CK; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Salmeron S; Department of Prosthodontics and Periodontics, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
  • de Carvalho ÉBS; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Liboa-Filho PN; Department of Physics, Faculty of Science, State University of São Paulo, Bauru, São Paulo, Brazil.
  • Damante CA; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Sant'Ana ACP; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Zangrando MSR; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • Greghi SLA; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
  • de Rezende MLR; Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Microsc Res Tech ; 82(7): 1004-1011, 2019 Jul.
Article em En | MEDLINE | ID: mdl-30839133
Previous studies have shown substances capable of similar effects of demineralization, accelerating the process of bone remodeling. This study investigated preosteoblasts behavior in cell culture after bone demineralization with citric acid and tetracycline. Seventy-four Wistar rats provided 144 calvarial bone samples, 126 of which were randomly divided in seven groups according to the treatment given to the surface: no demineralization (C), citric acid (CA), tetracycline (TCN) during 15, 30, and 60 s. Each group received preosteoblasts cultured for 24, 48, and 72 hr. Eighteen remaining samples were analyzed for the atomic percentage (A%) by energy dispersive spectroscopy (EDS) before and after demineralization. The average percentage of bone area covered by cells increased with time and it was significantly higher after 24 and 48 hr of culture in groups CA15s, CA30s, CA60s, TCN15s, and TCN30s than in groups TCN60 and C (p < 0.05). The cell morphology in all CA and TCN groups was shown to be compatible with more advanced stages of differentiation than in C group. The A% changed after demineralization. We conclude that demineralization with citric acid or tetracycline for 15-30 s increased the area of bone surface covered by preosteoblasts. The A% changes were not sufficient to impair the cells spreading and morphology. Bone demineralization may promote potential benefits in bone regenerative procedures. HIGHLIGHTS: Low pH effects did not interfere on cell growth. Bone demineralization favored the preosteoblasts growth. A possible alternative to improve graft consolidation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Crânio / Tetraciclina / Desmineralização Patológica Óssea / Ácido Cítrico Limite: Animals Idioma: En Revista: Microsc Res Tech Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Crânio / Tetraciclina / Desmineralização Patológica Óssea / Ácido Cítrico Limite: Animals Idioma: En Revista: Microsc Res Tech Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos