Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes.
Mol Biochem Parasitol
; 229: 62-74, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30831156
In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as ß-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite's glycosomes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Proteínas de Protozoários
/
Microcorpos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Mol Biochem Parasitol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Venezuela
País de publicação:
Holanda