Your browser doesn't support javascript.
loading
Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes.
Acosta, Héctor; Burchmore, Richard; Naula, Christina; Gualdrón-López, Melisa; Quintero-Troconis, Ender; Cáceres, Ana J; Michels, Paul A M; Concepción, Juan Luis; Quiñones, Wilfredo.
Afiliação
  • Acosta H; Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela.
  • Burchmore R; Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
  • Naula C; Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
  • Gualdrón-López M; Instituto Salud Global, Hospital Clinic-Universitat de Barcelona, and Institute for Health Sciences Trias i Pujol, Barcelona, Spain.
  • Quintero-Troconis E; Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela.
  • Cáceres AJ; Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela.
  • Michels PAM; Centre for Immunity, Infection and Evolution and Centre for Translational and Chemical Biology, The University of Edinburgh, Edinburgh, EH9 3FL, UK.
  • Concepción JL; Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela.
  • Quiñones W; Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela. Electronic address: wilqui@ula.ve.
Mol Biochem Parasitol ; 229: 62-74, 2019 04.
Article em En | MEDLINE | ID: mdl-30831156
In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as ß-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite's glycosomes.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Proteínas de Protozoários / Microcorpos Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Biochem Parasitol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Proteínas de Protozoários / Microcorpos Tipo de estudo: Prognostic_studies Idioma: En Revista: Mol Biochem Parasitol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Holanda