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Epigallocatechin-3-gallate protects the testis from damage generated by experimental cryptorchidism in rabbits.
Vigueras-Villaseñor, Rosa María; Jiménez Cabrera, Tania; Chávez Saldaña, Margarita; Jiménez Trejo, Francisco; Cuevas Alpuche, Osvaldo; Rojas-Castañeda, Julio César.
Afiliação
  • Vigueras-Villaseñor RM; Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
  • Jiménez Cabrera T; Department of Morphology, Faculty of Veterinary Medicine, National Autonomous University of Mexico, Mexico City, Mexico. rmvigueras@yahoo.com.mx.
  • Chávez Saldaña M; Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
  • Jiménez Trejo F; Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
  • Cuevas Alpuche O; Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
  • Rojas-Castañeda JC; Service of Urology, National Institute of Pediatrics, Mexico City, Mexico.
Histol Histopathol ; 34(8): 931-942, 2019 Aug.
Article em En | MEDLINE | ID: mdl-30829391
Cryptorchidism (CO) is a risk factor for infertility in men. It is associated with an increase in oxidative stress which alters the differentiation of the gonocytes to spermatogonia. Epigallocatechin-3-gallate (EGCG) is an antioxidant that acts as a free radical scavenger and activates the antioxidant enzymes. The aim of this work was to investigate if EGCG plays a role in the protection of the testicle from alterations generated by CO and its possible mechanism. Male rabbits 7 days old were divided into four groups and distributed as follows: 1) control (C) treated with EGCG vehicle (V) (C/V); 2) C with administration of EGCG from 65 to 120 days postpartum (dpp) (C/EGCG); 3) CO induced by administration of 17ß-estradiol plus EGCG vehicle (CO/V) and 4) CO plus EGCG administration (CO/EGCG). The animals were euthanized at 120 dpp and their testes were processed to evaluate lipid peroxidation, activities of superoxide dismutase (SOD) and catalase (CAT) enzymes as well as serum testosterone (T) concentrations. In addition, the rates of apoptosis, cell proliferation and histological alterations were determined. The CO/EGCG group showed a significant reduction in lipid peroxidation, a significant increase in the anti-oxidant enzyme activities and concentrations of T. Also, there was a significant decrease in the histological alterations, absence of gonocytes and active spermatogenesis when compared with CO/V group. These results show that EGCG reduces lipid peroxidation and increases the activity of the endogenous anti-oxidant system which protects the testes from alterations produced by oxidative stress generated during experimental CO.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Catequina / Criptorquidismo / Antioxidantes Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Histol Histopathol Assunto da revista: HISTOLOGIA / PATOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Espanha
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Catequina / Criptorquidismo / Antioxidantes Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Histol Histopathol Assunto da revista: HISTOLOGIA / PATOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Espanha