Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System.
Viruses
; 11(2)2019 02 22.
Article
em En
| MEDLINE
| ID: mdl-30813274
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella enteritidis
/
Fagos de Salmonella
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Genômica
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Interações entre Hospedeiro e Microrganismos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Viruses
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Colômbia
País de publicação:
Suíça