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Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors.
Haghighi, Malihe Fathi; Seyyedi, Noorossadat; Farhadi, Ali; Zare, Farahnaz; Kasraian, Leila; Refiei Dehbidi, Gholam Reza; Ranjbaran, Reza; Behzad-Behbahani, Abbas.
Afiliação
  • Haghighi MF; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran; Shiraz Islamic Azad University, Department of Microbiology, Shiraz, Iran.
  • Seyyedi N; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
  • Farhadi A; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
  • Zare F; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
  • Kasraian L; High Institute for Research and Education in Transfusion Medicine, Blood Transfusion Research Center, Shiraz, Iran.
  • Refiei Dehbidi GR; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
  • Ranjbaran R; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
  • Behzad-Behbahani A; Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran. Electronic address: behzadba@sums.ac.ir.
Braz J Infect Dis ; 23(1): 22-26, 2019.
Article em En | MEDLINE | ID: mdl-30807732
OBJECTIVES: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors. METHODS: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at -70°C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays. RESULTS: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses were found in 11 (4.4%) out of 250 blood samples. DISCUSSION: Our study provides important data on polyomavirus infection in peripheral blood mononuclear leukocytes in immunocompetent individuals. These data indicate significant differences between the prevalence of BKV and JCV infection in healthy blood donors. The prevalence of BK and JC virus infection is higher in the age range 30-39 years compared to other age ranges.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Tumorais por Vírus / Doadores de Sangue / Leucócitos Mononucleares / Vírus BK / Vírus JC / Infecções por Polyomavirus Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Tumorais por Vírus / Doadores de Sangue / Leucócitos Mononucleares / Vírus BK / Vírus JC / Infecções por Polyomavirus Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil